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| [[Image:2i2t.gif|left|200px]] | | #REDIRECT [[4v50]] This PDB entry is obsolete and replaced by 4v50 |
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| {{Structure
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| |PDB= 2i2t |SIZE=350|CAPTION= <scene name='initialview01'>2i2t</scene>, resolution 3.220Å
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| |SITE=
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| |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> and <scene name='pdbligand=MG:MAGNESIUM ION'>MG</scene>
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| |ACTIVITY=
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| |GENE=
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| }}
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| '''Crystal Structure of Ribosome with messenger RNA and the Anticodon stem-loop of P-site tRNA. This file contains the 50s subunit of one 70s ribosome. The entire crystal structure contains two 70s ribosomes and is described in remark 400.'''
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| ==Overview==
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| Protein synthesis requires the accurate positioning of mRNA and tRNA in the peptidyl-tRNA site of the ribosome. Here we describe x-ray crystal structures of the intact bacterial ribosome from Escherichia coli in a complex with mRNA and the anticodon stem-loop of P-site tRNA. At 3.5-A resolution, these structures reveal rearrangements in the intact ribosome that clamp P-site tRNA and mRNA on the small ribosomal subunit. Binding of the anticodon stem-loop of P-site tRNA to the ribosome is sufficient to lock the head of the small ribosomal subunit in a single conformation, thereby preventing movement of mRNA and tRNA before mRNA decoding.
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| ==About this Structure==
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| 2I2T is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2I2T OCA].
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| ==Reference==
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| Structural basis for mRNA and tRNA positioning on the ribosome., Berk V, Zhang W, Pai RD, Cate JH, Proc Natl Acad Sci U S A. 2006 Oct 24;103(43):15830-4. Epub 2006 Oct 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17038497 17038497]
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| [[Category: Escherichia coli]]
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| [[Category: Protein complex]]
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| [[Category: Berk, V.]]
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| [[Category: Cate, J H.D.]]
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| [[Category: Pai, R D.]]
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| [[Category: Zhang, W.]]
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| [[Category: MG]]
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| [[Category: ZN]]
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| [[Category: protein-nucleic acid complex]]
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| [[Category: ribosome]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:24:43 2008''
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