|
|
| (13 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| [[Image:2ii2.jpg|left|200px]]
| |
|
| |
|
| {{Structure
| | ==Crystal Structure of Alpha-11 Giardin== |
| |PDB= 2ii2 |SIZE=350|CAPTION= <scene name='initialview01'>2ii2</scene>, resolution 1.100Å
| | <StructureSection load='2ii2' size='340' side='right'caption='[[2ii2]], [[Resolution|resolution]] 1.10Å' scene=''> |
| |SITE= | | == Structural highlights == |
| |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
| | <table><tr><td colspan='2'>[[2ii2]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2II2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2II2 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.1Å</td></tr> |
| |GENE=
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| }}
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ii2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ii2 OCA], [https://pdbe.org/2ii2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ii2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ii2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ii2 ProSAT]</span></td></tr> |
| | | </table> |
| '''Crystal Structure of Alpha-11 Giardin''' | | == Function == |
| | | [https://www.uniprot.org/uniprot/Q4VPP2_GIAIN Q4VPP2_GIAIN] |
| | | == Evolutionary Conservation == |
| ==Overview== | | [[Image:Consurf_key_small.gif|200px|right]] |
| Alpha-11 giardin is a member of the multi-gene alpha-giardin family in the intestinal protozoan, Giardia lamblia. This gene family shares an ancestry with the annexin super family, whose common characteristic is calcium-dependent binding to membranes that contain acidic phospholipids. Several alpha giardins are highly expressed during parasite-induced diarrhea in humans. Despite being a member of a large family of proteins, little is known about the function and cellular localization of alpha-11 giardin, although giardins are often associated with the cytoskeleton. It has been shown that Giardia exhibits high levels of alpha-11 giardin mRNA transcript throughout its life cycle; however, constitutive over-expression of this protein is lethal to the parasite. Determining the three-dimensional structure of an alpha-giardin is essential to identifying functional domains shared in the alpha-giardin family. Here we report the crystal structures of the apo and Ca(2+)-bound forms of alpha-11 giardin, the first alpha giardin to be characterized structurally. Crystals of apo and Ca(2+)-bound alpha-11 giardin diffracted to 1.1 A and 2.93 A, respectively. The crystal structure of selenium-substituted apo alpha-11 giardin reveals a planar array of four tandem repeats of predominantly alpha-helical domains, reminiscent of previously determined annexin structures, making this the highest-resolution structure of an annexin to date. The apo alpha-11 giardin structure also reveals a hydrophobic core formed between repeats I/IV and II/III, a region typically hydrophilic in other annexins. Surprisingly, the Ca(2+)-bound structure contains only a single calcium ion, located in the DE loop of repeat I and coordinated differently from the two types of calcium sites observed in previous annexin structures. The apo and Ca(2+)-bound alpha-11 giardin structures assume overall similar conformations; however, Ca(2+)-bound alpha-11 giardin crystallized in a lower-symmetry space group with four molecules in the asymmetric unit. Vesicle-binding studies suggest that alpha-11 giardin, unlike most other annexins, does not bind to vesicles composed of acidic phospholipids in a calcium-dependent manner.
| | Check<jmol> |
| | | <jmolCheckbox> |
| ==About this Structure== | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ii/2ii2_consurf.spt"</scriptWhenChecked> |
| 2II2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Giardia_intestinalis Giardia intestinalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2II2 OCA].
| | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | | <text>to colour the structure by Evolutionary Conservation</text> |
| ==Reference==
| | </jmolCheckbox> |
| Apo and calcium-bound crystal structures of Alpha-11 giardin, an unusual annexin from Giardia lamblia., Pathuri P, Nguyen ET, Svard SG, Luecke H, J Mol Biol. 2007 Apr 27;368(2):493-508. Epub 2007 Feb 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17355882 17355882]
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ii2 ConSurf]. |
| | <div style="clear:both"></div> |
| | __TOC__ |
| | </StructureSection> |
| [[Category: Giardia intestinalis]] | | [[Category: Giardia intestinalis]] |
| [[Category: Single protein]] | | [[Category: Large Structures]] |
| [[Category: Luecke, H.]] | | [[Category: Luecke H]] |
| [[Category: Nguyen, E T.]] | | [[Category: Nguyen ET]] |
| [[Category: Pathuri, P.]] | | [[Category: Pathuri P]] |
| [[Category: SO4]]
| |
| [[Category: helix-turn-helix]]
| |
| | |
| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:29:51 2008''
| |
