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| [[Image:2ip2.jpg|left|200px]]
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| {{Structure
| | ==Structure of the Pyocyanin Biosynthetic Protein PhzM== |
| |PDB= 2ip2 |SIZE=350|CAPTION= <scene name='initialview01'>2ip2</scene>, resolution 1.80Å
| | <StructureSection load='2ip2' size='340' side='right'caption='[[2ip2]], [[Resolution|resolution]] 1.80Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[2ip2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IP2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2IP2 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| |GENE= phzM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 Pseudomonas aeruginosa])
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ip2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ip2 OCA], [https://pdbe.org/2ip2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ip2 RCSB], [https://www.ebi.ac.uk/pdbsum/2ip2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ip2 ProSAT]</span></td></tr> |
| }}
| | </table> |
| | | == Function == |
| '''Structure of the Pyocyanin Biosynthetic Protein PhzM''' | | [https://www.uniprot.org/uniprot/PHZM_PSEAE PHZM_PSEAE] Involved in the biosynthesis of pyocyanine, a blue-pigmented phenazine derivative, which plays a role in virulence. Converts phenazine-1-carboxylate (PCA) to 5-methylphenazine-1-carboxylate (5-methyl-PCA).<ref>PMID:17253782</ref> |
| | | == Evolutionary Conservation == |
| | | [[Image:Consurf_key_small.gif|200px|right]] |
| ==Overview== | | Check<jmol> |
| Pyocyanin is a biologically active phenazine produced by the human pathogen Pseudomonas aeruginosa. It is thought to endow P. aeruginosa with a competitive growth advantage in colonized tissue and is also thought to be a virulence factor in diseases such as cystic fibrosis and AIDS where patients are commonly infected by pathogenic Pseudomonads due to their immunocompromised state. Pyocyanin is also a chemically interesting compound due to its unusual oxidation-reduction activity. Phenazine-1-carboxylic acid, the precursor to the bioactive phenazines, is synthesized from chorismic acid by enzymes encoded in a seven-gene cistron in P. aeruginosa and in other Pseudomonads. Phenzine-1-carboxylic acid is believed to be converted to pyocyanin by the sequential actions of the putative S-adenosylmethionine-dependent N-methyltransferase PhzM and the putative flavin-dependent hydroxylase PhzS. Here we report the 1.8 A crystal structure of PhzM determined by single anomalous dispersion. Unlike many methyltransferases, PhzM is a dimer in solution. The 36 kDa PhzM polypeptide folds into three domains. The C-terminal domain exhibits the alpha/beta-hydrolase fold typical of small molecule methyltransferases. Two smaller N-terminal domains form much of the dimer interface. Structural alignments with known methyltransferases show that PhzM is most similar to the plant O-methyltransferases that are characterized by an unusual intertwined dimer interface. The structure of PhzM contains no ligands, and the active site is open and solvent-exposed when compared to structures of similar enzymes. In vitro experiments using purified PhzM alone demonstrate that it has little or no ability to methylate phenzine-1-carboxylic acid. However, when the putative hydroxylase PhzS is included, pyocyanin is readily produced. This observation suggests that a mechanism has evolved in P. aeruginosa that ensures efficient production of pyocyanin via the prevention of the formation and release of an unstable and potentially deleterious intermediate.
| | <jmolCheckbox> |
| | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ip/2ip2_consurf.spt"</scriptWhenChecked> |
| ==About this Structure== | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| 2IP2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2IP2 OCA].
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | | </jmolCheckbox> |
| ==Reference== | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ip2 ConSurf]. |
| Structural and functional analysis of the pyocyanin biosynthetic protein PhzM from Pseudomonas aeruginosa., Parsons JF, Greenhagen BT, Shi K, Calabrese K, Robinson H, Ladner JE, Biochemistry. 2007 Feb 20;46(7):1821-8. Epub 2007 Jan 25. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17253782 17253782]
| | <div style="clear:both"></div> |
| [[Category: Pseudomonas aeruginosa]] | | == References == |
| [[Category: Single protein]] | | <references/> |
| [[Category: Ladner, J E.]] | | __TOC__ |
| [[Category: Parsons, J F.]] | | </StructureSection> |
| [[Category: Robinson, H.]] | | [[Category: Large Structures]] |
| [[Category: Shi, K.]] | | [[Category: Pseudomonas aeruginosa PAO1]] |
| [[Category: methyltransferase]]
| | [[Category: Ladner JE]] |
| [[Category: phenazine]]
| | [[Category: Parsons JF]] |
| [[Category: phenazine-1-carboxylic acid]]
| | [[Category: Robinson H]] |
| [[Category: phzm]]
| | [[Category: Shi K]] |
| [[Category: pyocyanin]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:31:59 2008''
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