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==CRYSTAL STRUCTURE OF A BERENIL-DODECANUCLEOTIDE COMPLEX: THE ROLE OF WATER IN SEQUENCE-SPECIFIC LIGAND BINDING==
==CRYSTAL STRUCTURE OF A BERENIL-DODECANUCLEOTIDE COMPLEX: THE ROLE OF WATER IN SEQUENCE-SPECIFIC LIGAND BINDING==
<StructureSection load='2dbe' size='340' side='right' caption='[[2dbe]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
<StructureSection load='2dbe' size='340' side='right'caption='[[2dbe]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2dbe]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DBE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2DBE FirstGlance]. <br>
<table><tr><td colspan='2'>[[2dbe]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2DBE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2DBE FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BRN:BERENIL'>BRN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2dbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dbe OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2dbe RCSB], [http://www.ebi.ac.uk/pdbsum/2dbe PDBsum]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BRN:BERENIL'>BRN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2dbe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2dbe OCA], [https://pdbe.org/2dbe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2dbe RCSB], [https://www.ebi.ac.uk/pdbsum/2dbe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2dbe ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The three-dimensional structure of a complex between the dodecanucleotide d(CGCGAATTCGCG) and the anti-trypanocidal drug berenil, has been determined to a resolution of 2.5 A. The structure has been solved by molecular replacement and refined to an R factor of 0.177. A total of 49 water molecules have been located. The drug is bound at the 5'-AAT-3' region of the oligonucleotide. At one end of the drug the amidinium group is in hydrogen-bonded contact with N3 of the adenine base complementary to the thymine of the AAT. The other amidinium group does not make direct interactions with the DNA. Instead, a water molecule mediates between them. This is in hydrogen-bonded contact with an amidinium nitrogen atom, N3 of the 5' end adenine base and the ring oxygen atom of an adjacent deoxyribose. Molecular mechanics calculations have been performed on this complex, with the drug at various positions along the sequence. These show that the observed position is only 0.8 kcal/mol higher in energy than the best position. It is suggested that there is a broad energy well in the AATT region for this drug, and that water molecules as well as the neighbouring sequence, will determine precise positioning. More general aspects of minor groove binding are discussed.
Crystal structure of a berenil-dodecanucleotide complex: the role of water in sequence-specific ligand binding.,Brown DG, Sanderson MR, Skelly JV, Jenkins TC, Brown T, Garman E, Stuart DI, Neidle S EMBO J. 1990 Apr;9(4):1329-34. PMID:2323343<ref>PMID:2323343</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Brown, D G]]
[[Category: Large Structures]]
[[Category: Brown, T]]
[[Category: Brown DG]]
[[Category: Garman, E]]
[[Category: Brown T]]
[[Category: Jenkins, T C]]
[[Category: Garman E]]
[[Category: Neidle, S]]
[[Category: Jenkins TC]]
[[Category: Sanderson, M R]]
[[Category: Neidle S]]
[[Category: Skelly, J V]]
[[Category: Sanderson MR]]
[[Category: Stuart, D I]]
[[Category: Skelly JV]]
[[Category: B-dna]]
[[Category: Stuart DI]]
[[Category: Complexed with drug]]
[[Category: Dna]]
[[Category: Double helix]]

Latest revision as of 09:18, 14 February 2024

CRYSTAL STRUCTURE OF A BERENIL-DODECANUCLEOTIDE COMPLEX: THE ROLE OF WATER IN SEQUENCE-SPECIFIC LIGAND BINDING

2dbe, resolution 2.50Å

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