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==STRUCTURE OF TAB5 ALKALINE PHOSPHATASE MUTANT HIS 135 GLU WITH MG BOUND IN THE M3 SITE.==
 
<StructureSection load='2w5x' size='340' side='right' caption='[[2w5x]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
==Structure of TAB5 alkaline phosphatase mutant His 135 Glu with Mg bound in the M3 site.==
<StructureSection load='2w5x' size='340' side='right'caption='[[2w5x]], [[Resolution|resolution]] 1.99&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2w5x]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Antarctic_bacterium_tab5 Antarctic bacterium tab5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5X OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2W5X FirstGlance]. <br>
<table><tr><td colspan='2'>[[2w5x]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Antarctic_bacterium_TAB5 Antarctic bacterium TAB5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2W5X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2W5X FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.99&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2w5v|2w5v]], [[2w5w|2w5w]], [[2iuc|2iuc]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2w5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5x OCA], [https://pdbe.org/2w5x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2w5x RCSB], [https://www.ebi.ac.uk/pdbsum/2w5x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2w5x ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Alkaline_phosphatase Alkaline phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.1 3.1.3.1] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2w5x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2w5x OCA], [http://www.rcsb.org/pdb/explore.do?structureId=2w5x RCSB], [http://www.ebi.ac.uk/pdbsum/2w5x PDBsum]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9KWY4_UNCXX Q9KWY4_UNCXX]
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/2w5x_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/w5/2w5x_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2w5x ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 2w5x" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Alkaline phosphatase|Alkaline phosphatase]]
*[[Alkaline phosphatase 3D structures|Alkaline phosphatase 3D structures]]
*[[User:Andrzej Lyskowski|User:Andrzej Lyskowski]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Alkaline phosphatase]]
[[Category: Antarctic bacterium TAB5]]
[[Category: Antarctic bacterium tab5]]
[[Category: Large Structures]]
[[Category: Bouriotis, V]]
[[Category: Bouriotis V]]
[[Category: Feller, G]]
[[Category: Feller G]]
[[Category: Guthrie, E]]
[[Category: Guthrie E]]
[[Category: Heikinheimo, P]]
[[Category: Heikinheimo P]]
[[Category: Koutsioulis, D]]
[[Category: Koutsioulis D]]
[[Category: Lyskowski, A]]
[[Category: Lyskowski A]]
[[Category: Maki, S]]
[[Category: Maki S]]
[[Category: Cold adaptation]]
[[Category: Hydrolase]]
[[Category: Psychrophile]]

Latest revision as of 08:01, 23 October 2024

Structure of TAB5 alkaline phosphatase mutant His 135 Glu with Mg bound in the M3 site.

2w5x, resolution 1.99Å

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