2o8p: Difference between revisions
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==Crystal structure of a putative 14-3-3 protein from Cryptosporidium parvum, cgd7_2470== | |||
<StructureSection load='2o8p' size='340' side='right'caption='[[2o8p]], [[Resolution|resolution]] 1.82Å' scene=''> | |||
| | == Structural highlights == | ||
| | <table><tr><td colspan='2'>[[2o8p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cryptosporidium_parvum Cryptosporidium parvum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2O8P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2O8P FirstGlance]. <br> | ||
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.82Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2o8p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2o8p OCA], [https://pdbe.org/2o8p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2o8p RCSB], [https://www.ebi.ac.uk/pdbsum/2o8p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2o8p ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q5CYG0_CRYPI Q5CYG0_CRYPI] | |||
== Evolutionary Conservation == | |||
[[Image:Consurf_key_small.gif|200px|right]] | |||
Check<jmol> | |||
<jmolCheckbox> | |||
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/o8/2o8p_consurf.spt"</scriptWhenChecked> | |||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> | |||
<text>to colour the structure by Evolutionary Conservation</text> | |||
</jmolCheckbox> | |||
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2o8p ConSurf]. | |||
<div style="clear:both"></div> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The parasite Cryptosporidium parvum has three 14-3-3 proteins: Cp14epsilon, Cp14a and Cp14b, with only Cp14epsilon similar to human 14-3-3 proteins in sequence, peptide-binding properties and structure. Structurally, Cp14a features the classical 14-3-3 dimer but with a uniquely wide pocket and a disoriented RRY triad potentially incapable of binding phosphopeptides. The Cp14b protein deviates from the norm significantly: (i) In one subunit, the phosphorylated C-terminal tail is bound in the binding groove like a phosphopeptide. This supports our binding study indicating this protein was stabilized by a peptide mimicking its last six residues. (ii) The other subunit has eight helices instead of nine, with alphaA and alphaB forming a single helix and occluding the peptide-binding cleft. (iii) The protein forms a degenerate dimer with the two binding grooves divided and facing opposite directions. These features conspire to block and disrupt the bicameral substrate-binding pocket, suggesting a possible tripartite auto-regulation mechanism that has not been observed previously. ENHANCED VERSION: This article can also be viewed as an enhanced version in which the text of the article is integrated with interactive 3D representations and animated transitions. Please note that a web plugin is required to access this enhanced functionality. Instructions for the installation and use of the web plugin are available in Text S1. | |||
Characterization of 14-3-3 proteins from Cryptosporidium parvum.,Brokx SJ, Wernimont AK, Dong A, Wasney GA, Lin YH, Lew J, Vedadi M, Lee WH, Hui R PLoS One. 2011;6(8):e14827. Epub 2011 Aug 11. PMID:21853016<ref>PMID:21853016</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 2o8p" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Cryptosporidium parvum]] | [[Category: Cryptosporidium parvum]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Arrowsmith | [[Category: Arrowsmith CH]] | ||
[[Category: Bochkarev | [[Category: Bochkarev A]] | ||
[[Category: Brokx | [[Category: Brokx SJ]] | ||
[[Category: Dong | [[Category: Dong A]] | ||
[[Category: Edwards | [[Category: Edwards AM]] | ||
[[Category: Hassanali | [[Category: Hassanali A]] | ||
[[Category: Hui | [[Category: Hui R]] | ||
[[Category: Kozieradzki | [[Category: Kozieradzki I]] | ||
[[Category: Lew | [[Category: Lew J]] | ||
[[Category: Lin | [[Category: Lin L]] | ||
[[Category: Sundstrom M]] | |||
[[Category: Sundstrom | [[Category: Vedadi M]] | ||
[[Category: Vedadi | [[Category: Walker JR]] | ||
[[Category: Walker | [[Category: Wasney G]] | ||
[[Category: Wasney | [[Category: Weigelt J]] | ||
[[Category: Weigelt | [[Category: Zhao Y]] | ||
[[Category: Zhao | |||
Latest revision as of 01:15, 21 November 2024
Crystal structure of a putative 14-3-3 protein from Cryptosporidium parvum, cgd7_2470
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