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[[Image:2ong.gif|left|200px]]


{{Structure
==Crystal Structure of of limonene synthase with 2-fluorogeranyl diphosphate (FGPP).==
|PDB= 2ong |SIZE=350|CAPTION= <scene name='initialview01'>2ong</scene>, resolution 2.7&Aring;
<StructureSection load='2ong' size='340' side='right'caption='[[2ong]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=FPG:(1S)-1-[(1R)-1-FLUOROETHYL]-1,5-DIMETHYLHEXYL+TRIHYDROGEN+DIPHOSPHATE'>FPG</scene> and <scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>
<table><tr><td colspan='2'>[[2ong]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mentha_spicata Mentha spicata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ONG FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTB:2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>BTB</scene>, <scene name='pdbligand=FPG:(1S)-1-[(1R)-1-FLUOROETHYL]-1,5-DIMETHYLHEXYL+TRIHYDROGEN+DIPHOSPHATE'>FPG</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ong FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ong OCA], [https://pdbe.org/2ong PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ong RCSB], [https://www.ebi.ac.uk/pdbsum/2ong PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ong ProSAT]</span></td></tr>
 
</table>
'''Crystal Structure of of limonene synthase with 2-fluorogeranyl diphosphate (FGPP).'''
== Function ==
 
[https://www.uniprot.org/uniprot/Q40322_MENSP Q40322_MENSP]
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/on/2ong_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ong ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The crystal structure of (4S)-limonene synthase from Mentha spic ata, a metal ion-dependent monoterpene cyclase that catalyzes the coupled isomerization and cyclization of geranyl diphosphate, is reported at 2.7-A; resolution in two forms liganded to the substrate and intermediate analogs, 2-fluorogeranyl diphosphate and 2-fluorolinalyl diphosphate, respectively. The implications of these findings are described for domain interactions in the homodimer and for changes in diphosphate-metal ion coordination and substrate binding conformation in the course of the multistep reaction.
The crystal structure of (4S)-limonene synthase from Mentha spic ata, a metal ion-dependent monoterpene cyclase that catalyzes the coupled isomerization and cyclization of geranyl diphosphate, is reported at 2.7-A; resolution in two forms liganded to the substrate and intermediate analogs, 2-fluorogeranyl diphosphate and 2-fluorolinalyl diphosphate, respectively. The implications of these findings are described for domain interactions in the homodimer and for changes in diphosphate-metal ion coordination and substrate binding conformation in the course of the multistep reaction.


==About this Structure==
Structure of limonene synthase, a simple model for terpenoid cyclase catalysis.,Hyatt DC, Youn B, Zhao Y, Santhamma B, Coates RM, Croteau RB, Kang C Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5360-5. Epub 2007 Mar 19. PMID:17372193<ref>PMID:17372193</ref>
2ONG is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Mentha_spicata Mentha spicata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ONG OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structure of limonene synthase, a simple model for terpenoid cyclase catalysis., Hyatt DC, Youn B, Zhao Y, Santhamma B, Coates RM, Croteau RB, Kang C, Proc Natl Acad Sci U S A. 2007 Mar 27;104(13):5360-5. Epub 2007 Mar 19. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17372193 17372193]
</div>
<div class="pdbe-citations 2ong" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Mentha spicata]]
[[Category: Mentha spicata]]
[[Category: Single protein]]
[[Category: Croteau R]]
[[Category: Croteau, R.]]
[[Category: Hyatt DC]]
[[Category: Hyatt, D C.]]
[[Category: Kang C]]
[[Category: Kang, C.]]
[[Category: Youn B]]
[[Category: Youn, B.]]
[[Category: BTB]]
[[Category: FPG]]
[[Category: MN]]
[[Category: 2 fluorogeranyl diphosphate]]
[[Category: 2-fluorolinalyl diphosphate]]
[[Category: crystal structure]]
[[Category: geranyl diphosphate]]
[[Category: linalyl diphosphate]]
[[Category: monoterpene cyclase]]
[[Category: monoterpene synthase]]
 
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Retrieved from "https://proteopedia.org/2ong"