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==Crystal structure analysis of trioxacarcin A covalently bound to d(AACCGGTT)==
==Crystal structure analysis of trioxacarcin A covalently bound to d(AACCGGTT)==
<StructureSection load='3c2j' size='340' side='right' caption='[[3c2j]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
<StructureSection load='3c2j' size='340' side='right'caption='[[3c2j]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3c2j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C2J OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3C2J FirstGlance]. <br>
<table><tr><td colspan='2'>[[3c2j]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3C2J OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3C2J FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GGT:TRIOXACARCIN+A'>GGT</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3c2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c2j OCA], [http://www.rcsb.org/pdb/explore.do?structureId=3c2j RCSB], [http://www.ebi.ac.uk/pdbsum/3c2j PDBsum]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GGT:TRIOXACARCIN+A'>GGT</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3c2j FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3c2j OCA], [https://pdbe.org/3c2j PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3c2j RCSB], [https://www.ebi.ac.uk/pdbsum/3c2j PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3c2j ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We report a crystal structure that shows an antibiotic that extracts a nucleobase from a DNA molecule 'caught in the act' after forming a covalent bond but before departing with the base. The structure of trioxacarcin A covalently bound to double-stranded d(AACCGGTT) was determined to 1.78 A resolution by MAD phasing employing brominated oligonucleotides. The DNA-drug complex has a unique structure that combines alkylation (at the N7 position of a guanine), intercalation (on the 3'-side of the alkylated guanine), and base flip-out. An antibiotic-induced flipping-out of a single, nonterminal nucleobase from a DNA duplex was observed for the first time in a crystal structure.
Crystal structure of trioxacarcin A covalently bound to DNA.,Pfoh R, Laatsch H, Sheldrick GM Nucleic Acids Res. 2008 May 3;. PMID:18453630<ref>PMID:18453630</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Pfoh, R]]
[[Category: Large Structures]]
[[Category: Sheldrick, G M]]
[[Category: Pfoh R]]
[[Category: B-dna]]
[[Category: Sheldrick GM]]
[[Category: Base flipping]]
[[Category: Dna]]
[[Category: Dna-drug complex]]
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