4s21: Difference between revisions
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==Crystal structure of the photosensory core module of bacteriophytochrome RPA3015 from R. palustris== | |||
<StructureSection load='4s21' size='340' side='right'caption='[[4s21]], [[Resolution|resolution]] 3.25Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4s21]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodopseudomonas_palustris_CGA009 Rhodopseudomonas palustris CGA009]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4S21 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4S21 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.25Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BLA:BILIVERDINE+IX+ALPHA'>BLA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4s21 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4s21 OCA], [https://pdbe.org/4s21 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4s21 RCSB], [https://www.ebi.ac.uk/pdbsum/4s21 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4s21 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q6N5G3_RHOPA Q6N5G3_RHOPA] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
RpBphP2 and RpBphP3, two tandem bacteriophytochromes from the photosynthetic bacterium Rhodopseudomonas palustris, share high sequence identity but exhibit distinct photoconversion behavior. Unlike the canonical RpBphP2, RpBphP3 photoconverts to an unusual near-red-absorbing (Pnr) state; both are required for synthesis of light-harvesting complexes under low-light conditions. Here we report the crystal structures of the photosensory core modules of RpBphP2 and RpBphP3. Despite different quaternary structures, RpBphP2 and RpBphP3 adopt nearly identical tertiary structures. The RpBphP3 structure reveals tongue-and-groove interactions at the interface between the GAF and PHY domains. A single mutation in the PRxSF motif at the GAF-PHY interface abolishes light-induced formation of the Pnr state in RpBphP3, possibly due to altered structural rigidity of the chromophore-binding pocket. Structural comparisons suggest that long-range signaling involves structural rearrangement of the helical spine at the dimer interface. These structures, together with mutational studies, provide insights into photoconversion and the long-range signaling mechanism in phytochromes. | |||
Light Signaling Mechanism of Two Tandem Bacteriophytochromes.,Yang X, Stojkovic EA, Ozarowski WB, Kuk J, Davydova E, Moffat K Structure. 2015 Jul 7;23(7):1179-89. doi: 10.1016/j.str.2015.04.022. Epub 2015, Jun 18. PMID:26095026<ref>PMID:26095026</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 4s21" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Rhodopseudomonas palustris CGA009]] | |||
[[Category: Moffat K]] | |||
[[Category: Ozarowski WB]] | |||
[[Category: Stojkovi EA]] | |||
[[Category: Yang X]] | |||
Latest revision as of 18:01, 20 September 2023
Crystal structure of the photosensory core module of bacteriophytochrome RPA3015 from R. palustris
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