4oun: Difference between revisions
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==Crystal Structure of Mini-ribonuclease 3 from Bacillus subtilis== | ==Crystal Structure of Mini-ribonuclease 3 from Bacillus subtilis== | ||
<StructureSection load='4oun' size='340' side='right' caption='[[4oun]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='4oun' size='340' side='right'caption='[[4oun]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4oun]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OUN OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[4oun]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OUN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OUN FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oun FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oun OCA], [https://pdbe.org/4oun PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oun RCSB], [https://www.ebi.ac.uk/pdbsum/4oun PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oun ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/MRNC_BACSU MRNC_BACSU] Involved in correct processing of both the 5' and 3' ends of 23S rRNA precursor. Processes 30S rRNA precursor transcript even in absence of ribonuclease 3 (Rnc); Rnc processes 30S rRNA into smaller rRNA precursors. Cleaves more efficiently on assembled 50S ribosomal subunits. Cleavage is strongly stimulated by ribosomal protein L3 (RplC); 20-30% DMSO can replace RplC, suggesting RplC may alter rRNA conformation.<ref>PMID:18363798</ref> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Ribonucleases (RNases) play a critical role in RNA processing and degradation by hydrolyzing phosphodiester bonds (exo- or endonucleolytically). Many RNases that cut RNA internally exhibit substrate specificity, but their target sites are usually limited to one or a few specific nucleotides in single-stranded RNA and often in a context of a particular three-dimensional structure of the substrate. Thus far, no RNase counterparts of restriction enzymes have been identified which could cleave double-stranded RNA (dsRNA) in a sequence-specific manner. Here, we present evidence for a sequence-dependent cleavage of long dsRNA by RNase Mini-III from Bacillus subtilis (BsMiniIII). Analysis of the sites cleaved by this enzyme in limited digest of bacteriophage Phi6 dsRNA led to the identification of a consensus target sequence. We defined nucleotide residues within the preferred cleavage site that affected the efficiency of the cleavage and were essential for the discrimination of cleavable versus non-cleavable dsRNA sequences. We have also determined that the loop alpha5b-alpha6, a distinctive structural element in Mini-III RNases, is crucial for the specific cleavage, but not for dsRNA binding. Our results suggest that BsMiniIII may serve as a prototype of a sequence-specific dsRNase that could possibly be used for targeted cleavage of dsRNA. | |||
Sequence-specific cleavage of dsRNA by Mini-III RNase.,Glow D, Pianka D, Sulej AA, Kozlowski LP, Czarnecka J, Chojnowski G, Skowronek KJ, Bujnicki JM Nucleic Acids Res. 2015 Mar 11;43(5):2864-73. doi: 10.1093/nar/gkv009. Epub 2015 , Jan 29. PMID:25634891<ref>PMID:25634891</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 4oun" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ribonuclease 3D structures|Ribonuclease 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bujnicki | [[Category: Bacillus subtilis subsp. subtilis str. 168]] | ||
[[Category: Chojnowski | [[Category: Large Structures]] | ||
[[Category: Czarnecka | [[Category: Bujnicki JM]] | ||
[[Category: Glow | [[Category: Chojnowski G]] | ||
[[Category: Nowak | [[Category: Czarnecka J]] | ||
[[Category: Nowotny | [[Category: Glow D]] | ||
[[Category: Pianka | [[Category: Nowak E]] | ||
[[Category: Sabala | [[Category: Nowotny M]] | ||
[[Category: Skowronek | [[Category: Pianka D]] | ||
[[Category: Sabala I]] | |||
[[Category: Skowronek K]] | |||
