4xyh: Difference between revisions
From Proteopedia
Jump to navigationJump to search
No edit summary |
No edit summary |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Wild-type full length Mis16 in Schizosaccharomyces japonicus== | |||
<StructureSection load='4xyh' size='340' side='right'caption='[[4xyh]], [[Resolution|resolution]] 2.30Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4xyh]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_japonicus_yFS275 Schizosaccharomyces japonicus yFS275]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4XYH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4XYH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4xyh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4xyh OCA], [https://pdbe.org/4xyh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4xyh RCSB], [https://www.ebi.ac.uk/pdbsum/4xyh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4xyh ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/B6K598_SCHJY B6K598_SCHJY] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CENP-A is a centromere-specific histone H3 variant that is required for kinetochore assembly and accurate chromosome segregation. For it to function properly, CENP-A must be specifically localized to centromeres. In fission yeast, Scm3sp and the Mis18 complex, composed of Mis16, Eic1, and Mis18, function as a CENP-A(Cnp1)-specific chaperone and a recruiting factor, respectively, and together ensure accurate delivery of CENP-A(Cnp1) to centromeres. Although how Scm3sp specifically recognizes CENP-A(Cnp1) has been revealed recently, the recruiting mechanism of CENP-A(Cnp1) via the Mis18 complex remains unknown. In this study, we have determined crystal structures of Schizosaccharomyces japonicus Mis16 alone and in complex with the helix 1 of histone H4 (H4alpha1). Crystal structures followed by mutant analysis and affinity pull-downs have revealed that Mis16 recognizes both H4alpha1 and Scm3sp independently within the CENP-A(Cnp1)/H4:Scm3sp complex. This observation suggests that Mis16 gains CENP-A(Cnp1) specificity by recognizing both Scm3sp and histone H4. Our studies provide insights into the molecular mechanisms underlying specific recruitment of CENP-A(Cnp1)/H4:Scm3sp into centromeres. | |||
Mis16 Independently Recognizes Histone H4 and the CENP-ACnp1-Specific Chaperone Scm3sp.,An S, Kim H, Cho US J Mol Biol. 2015 Oct 9;427(20):3230-40. doi: 10.1016/j.jmb.2015.08.022. Epub 2015, Sep 4. PMID:26343758<ref>PMID:26343758</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: An | <div class="pdbe-citations 4xyh" style="background-color:#fffaf0;"></div> | ||
[[Category: Cho | == References == | ||
[[Category: Kim | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Schizosaccharomyces japonicus yFS275]] | |||
[[Category: An S]] | |||
[[Category: Cho U-S]] | |||
[[Category: Kim H]] | |||
Latest revision as of 07:52, 27 September 2023
Wild-type full length Mis16 in Schizosaccharomyces japonicus
| ||||||||||||
