4yhh: Difference between revisions
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New page: '''Unreleased structure''' The entry 4yhh is ON HOLD Authors: Schedlbauer, A., Kaminishi, T., Ochoa-Lizarralde, B., Dhimole, N., Zhou, S., Lopez-Alonso, J.P., Connell, S.R., Fucini, P. ... |
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==Crystal structure of the 30S ribosomal subunit from Thermus thermophilus in complex with tigecycline== | |||
<StructureSection load='4yhh' size='340' side='right'caption='[[4yhh]], [[Resolution|resolution]] 3.42Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4yhh]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YHH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YHH FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.417Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=T1C:TIGECYCLINE'>T1C</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yhh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yhh OCA], [https://pdbe.org/4yhh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yhh RCSB], [https://www.ebi.ac.uk/pdbsum/4yhh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yhh ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RS2_THET8 RS2_THET8] Spans the head-body hinge region of the 30S subunit. Is loosely associated with the 30S subunit.[HAMAP-Rule:MF_00291_B] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Although both tetracycline and tigecycline inhibit protein synthesis by sterically hindering the binding of tRNA to the ribosomal A-site, tigecycline shows increased efficacy in both in vitro and in vivo activity assays and escapes the most common resistance mechanisms associated with the tetracycline class of antibiotics. These differences in activities are attributed to the tert-butyl-glycylamido side chain found in tigecycline. Our structural analysis by X-ray crystallography shows that tigecycline binds the bacterial 30S ribosomal subunit with its tail in an extended conformation and makes extensive interactions with the 16S rRNA nucleotide C1054. These interactions would restrict the mobility of C1054 and contribute to the antimicrobial activity of tigecycline including its resistance to the ribosomal protection proteins. | |||
Structural characterization of an alternative-binding mode for tigecycline to the bacterial ribosome.,Schedlbauer A, Kaminishi T, Ochoa-Lizarralde B, Dhimole N, Zhou S, Lopez-Alonso JP, Connell SR, Fucini P Antimicrob Agents Chemother. 2015 Mar 9. pii: AAC.04895-14. PMID:25753625<ref>PMID:25753625</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[ | </div> | ||
[[Category: | <div class="pdbe-citations 4yhh" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: Connell | ==See Also== | ||
[[Category: | *[[Ribosomal protein THX 3D structures|Ribosomal protein THX 3D structures]] | ||
[[Category: Fucini | == References == | ||
[[Category: Kaminishi | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: Ochoa-Lizarralde | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Thermus thermophilus HB8]] | |||
[[Category: Connell SR]] | |||
[[Category: Dhimole N]] | |||
[[Category: Fucini P]] | |||
[[Category: Kaminishi T]] | |||
[[Category: Lopez-Alonso JP]] | |||
[[Category: Ochoa-Lizarralde B]] | |||
[[Category: Schedlbauer A]] | |||
[[Category: Zhou S]] | |||
Latest revision as of 06:57, 17 October 2024
Crystal structure of the 30S ribosomal subunit from Thermus thermophilus in complex with tigecycline
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