2n0r: Difference between revisions
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New page: '''Unreleased structure''' The entry 2n0r is ON HOLD Authors: Marchanka, A., Simon, B., Althoff-Ospelt, G., Carlomagno, T. Description: RNA structure determination by solid-state NMR s... |
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==RNA structure determination by solid-state NMR spectroscopy== | |||
<StructureSection load='2n0r' size='340' side='right'caption='[[2n0r]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2n0r]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N0R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N0R FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n0r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n0r OCA], [https://pdbe.org/2n0r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n0r RCSB], [https://www.ebi.ac.uk/pdbsum/2n0r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n0r ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Knowledge of the RNA three-dimensional structure, either in isolation or as part of RNP complexes, is fundamental to understand the mechanism of numerous cellular processes. Because of its flexibility, RNA represents a challenge for crystallization, while the large size of cellular complexes brings solution-state NMR to its limits. Here, we demonstrate an alternative approach on the basis of solid-state NMR spectroscopy. We develop a suite of experiments and RNA labeling schemes and demonstrate for the first time that ssNMR can yield a RNA structure at high-resolution. This methodology allows structural analysis of segmentally labelled RNA stretches in high-molecular weight cellular machines-independent of their ability to crystallize- and opens the way to mechanistic studies of currently difficult-to-access RNA-protein assemblies. | |||
RNA structure determination by solid-state NMR spectroscopy.,Marchanka A, Simon B, Althoff-Ospelt G, Carlomagno T Nat Commun. 2015 May 11;6:7024. doi: 10.1038/ncomms8024. PMID:25960310<ref>PMID:25960310</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Althoff-Ospelt | <div class="pdbe-citations 2n0r" style="background-color:#fffaf0;"></div> | ||
[[Category: Carlomagno | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Althoff-Ospelt G]] | |||
[[Category: Carlomagno T]] | |||
[[Category: Marchanka A]] | |||
[[Category: Simon B]] | |||
