4ypl: Difference between revisions

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'''Unreleased structure'''


The entry 4ypl is ON HOLD
==Crystal structure of a hexameric LonA protease bound to three ADPs==
<StructureSection load='4ypl' size='340' side='right'caption='[[4ypl]], [[Resolution|resolution]] 3.45&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4ypl]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Meiothermus_taiwanensis Meiothermus taiwanensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YPL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YPL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.45&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=4KZ:N-[(1R)-1-(DIHYDROXYBORANYL)-2-PHENYLETHYL]-NALPHA-(PYRAZIN-2-YLCARBONYL)-L-PHENYLALANINAMIDE'>4KZ</scene>, <scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ypl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ypl OCA], [https://pdbe.org/4ypl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ypl RCSB], [https://www.ebi.ac.uk/pdbsum/4ypl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ypl ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/A0A059VAZ3_9DEIN A0A059VAZ3_9DEIN] ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.[HAMAP-Rule:MF_01973]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Lon AAA+ protease (LonA) is an evolutionarily conserved protease that couples the ATPase cycle into motion to drive substrate translocation and degradation. A hallmark feature shared by AAA+ proteases is the stimulation of ATPase activity by substrates. Here we report the structure of LonA bound to three ADPs, revealing the first AAA+ protease assembly where the six protomers are arranged alternately in nucleotide-free and bound states. Nucleotide binding induces large coordinated movements of conserved pore loops from two pairs of three non-adjacent protomers and shuttling of the proteolytic groove between the ATPase site and a previously unknown Arg paddle. Structural and biochemical evidence supports the roles of the substrate-bound proteolytic groove in allosteric stimulation of ATPase activity and the conserved Arg paddle in driving substrate degradation. Altogether, this work provides a molecular framework for understanding how ATP-dependent chemomechanical movements drive allosteric processes for substrate degradation in a major protein-destruction machine.


Authors: Chien-Chu Lin, Chung-I Chang
Structural Insights into the Allosteric Operation of the Lon AAA+ Protease.,Lin CC, Su SC, Su MY, Liang PH, Feng CC, Wu SH, Chang CI Structure. 2016 May 3;24(5):667-75. doi: 10.1016/j.str.2016.03.001. Epub 2016 Mar, 31. PMID:27041592<ref>PMID:27041592</ref>


Description: The crystal structure of 3-helix bundle, AAA+ and protease domain of MtaLonA mutant -E423Q complex with ADP and MMH8709 (3HAAAP -E423Q -Mg2+ -ADP -MMH8709)
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Chien-Chu Lin, Chung-I Chang]]
<div class="pdbe-citations 4ypl" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Meiothermus taiwanensis]]
[[Category: Chang C-I]]
[[Category: Lin C-C]]
Retrieved from "https://proteopedia.org/4ypl"