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==Structure of a thermostable elevenfold mutant of limonene epoxide hydrolase from Rhodococcus erythropolis, containing two stabilizing disulfide bonds==
==Structure of a thermostable elevenfold mutant of limonene epoxide hydrolase from Rhodococcus erythropolis, containing two stabilizing disulfide bonds==
<StructureSection load='4r9l' size='340' side='right' caption='[[4r9l]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='4r9l' size='340' side='right'caption='[[4r9l]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4r9l]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R9L OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4R9L FirstGlance]. <br>
<table><tr><td colspan='2'>[[4r9l]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Rhodococcus_erythropolis Rhodococcus erythropolis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4R9L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4R9L FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=HYH:(2R)-2-HYDROXYHEXANAMIDE'>HYH</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1nww|1nww]], [[1nu3|1nu3]], [[4r9k|4r9k]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HYH:(2R)-2-HYDROXYHEXANAMIDE'>HYH</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Limonene-1,2-epoxide_hydrolase Limonene-1,2-epoxide hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.3.2.8 3.3.2.8] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4r9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r9l OCA], [https://pdbe.org/4r9l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4r9l RCSB], [https://www.ebi.ac.uk/pdbsum/4r9l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4r9l ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4r9l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4r9l OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4r9l RCSB], [http://www.ebi.ac.uk/pdbsum/4r9l PDBsum]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/LIMA_RHOER LIMA_RHOER]] Catalyzes the conversion of limonene-1,2-epoxide to limonene-1,2-diol. Can use both the (-) and (+) isomers of limonene-1,2-epoxide as substrates and also has some activity with 1-methylcyclohexene oxide, cyclohexene oxide and indene oxide as substrates.  
[https://www.uniprot.org/uniprot/LIMA_RHOER LIMA_RHOER] Catalyzes the conversion of limonene-1,2-epoxide to limonene-1,2-diol. Can use both the (-) and (+) isomers of limonene-1,2-epoxide as substrates and also has some activity with 1-methylcyclohexene oxide, cyclohexene oxide and indene oxide as substrates.
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
</div>
<div class="pdbe-citations 4r9l" style="background-color:#fffaf0;"></div>
==See Also==
*[[Epoxide hydrolase 3D structures|Epoxide hydrolase 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Limonene-1,2-epoxide hydrolase]]
[[Category: Large Structures]]
[[Category: Dijkstra, B W]]
[[Category: Rhodococcus erythropolis]]
[[Category: Floor, R J]]
[[Category: Dijkstra BW]]
[[Category: Janssen, D B]]
[[Category: Floor RJ]]
[[Category: Jekel, P A]]
[[Category: Janssen DB]]
[[Category: Scheltinga, A C.Terwisscha van]]
[[Category: Jekel PA]]
[[Category: Wijma, H J]]
[[Category: Terwisscha van Scheltinga AC]]
[[Category: Engineered]]
[[Category: Wijma HJ]]
[[Category: Hydrolase]]
[[Category: Limonene epoxide hydrolase]]
[[Category: Ntf-2 fold]]
[[Category: Thermostable]]