4yt3: Difference between revisions
From Proteopedia
Jump to navigationJump to search
New page: '''Unreleased structure''' The entry 4yt3 is ON HOLD until Paper Publication Authors: janocha, S., carius, y., bernhardt, r., lancaster, c.r.d. Description: CYP106A2 [[Category: Unrele... |
No edit summary |
||
| (5 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==CYP106A2== | |||
<StructureSection load='4yt3' size='340' side='right'caption='[[4yt3]], [[Resolution|resolution]] 1.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4yt3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Priestia_megaterium Priestia megaterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4YT3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4YT3 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4yt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4yt3 OCA], [https://pdbe.org/4yt3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4yt3 RCSB], [https://www.ebi.ac.uk/pdbsum/4yt3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4yt3 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/CPXM_PRIMG CPXM_PRIMG] Has the capacity to hydroxylate certain steroids in the 15-beta position. Also hydroxylates progesterone in the 11-alpha and 9-beta position.<ref>PMID:18481342</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
CYP106A2 from Bacillus megaterium ATCC 13368 is known as a bacterial steroid hydroxylase which is also capable to hydroxylate a variety of terpenoids. To further analyze the substrate specificity of this enzyme, different resin acids of the abietane- and pimarane-type were tested towards binding and conversion. Product formation could be shown for all tested substrates. Spectroscopic studies revealed type-I binding spectra for isopimaric acid but dehydroabietic acid did not induce a high-spin shift of the enzyme. Interestingly, binding of abietic acid resulted in a type-II difference spectrum typical for nitrogenous inhibitors. Co-crystallization of CYP106A2 with abietic acid and structure determination revealed a bending of the heme-cofactor when abietic acid was bound in the active site. Quantum chemical calculations strongly suggest that this heme distortion is the cause of the unusual spectroscopic characteristics. | |||
Crystal structure of CYP106A2 in substrate-free and substrate-bound form.,Janocha S, Carius Y, Hutter M, Lancaster CR, Bernhardt R Chembiochem. 2016 Feb 11. doi: 10.1002/cbic.201500524. PMID:26864272<ref>PMID:26864272</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 4yt3" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: | ==See Also== | ||
[[Category: Janocha | *[[Cytochrome P450 3D structures|Cytochrome P450 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Priestia megaterium]] | |||
[[Category: Bernhardt r]] | |||
[[Category: Carius y]] | |||
[[Category: Janocha S]] | |||
[[Category: Lancaster crd]] | |||
