2rcu: Difference between revisions

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[[Image:2rcu.gif|left|200px]]


{{Structure
==Crystal structure of rat carnitine palmitoyltransferase 2 in complex with r-3-(hexadecanoylamino)-4-(trimethylazaniumyl)butanoate==
|PDB= 2rcu |SIZE=350|CAPTION= <scene name='initialview01'>2rcu</scene>, resolution 1.78&Aring;
<StructureSection load='2rcu' size='340' side='right'caption='[[2rcu]], [[Resolution|resolution]] 1.78&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene> and <scene name='pdbligand=BUJ:(3R)-3-(hexadecanoylamino)-4-(trimethylammonio)butanoate'>BUJ</scene>
<table><tr><td colspan='2'>[[2rcu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2RCU FirstGlance]. <br>
|ACTIVITY= [http://en.wikipedia.org/wiki/Carnitine_O-palmitoyltransferase Carnitine O-palmitoyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.21 2.3.1.21]  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.78&#8491;</td></tr>
|GENE= Cpt2, Cpt-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BOG:B-OCTYLGLUCOSIDE'>BOG</scene>, <scene name='pdbligand=BUJ:(3R)-3-(HEXADECANOYLAMINO)-4-(TRIMETHYLAMMONIO)BUTANOATE'>BUJ</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2rcu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rcu OCA], [https://pdbe.org/2rcu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2rcu RCSB], [https://www.ebi.ac.uk/pdbsum/2rcu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2rcu ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/CPT2_RAT CPT2_RAT]  
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/rc/2rcu_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2rcu ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The mitochondrial membrane-associated carnitine palmitoyltransferase system is a validated target for the treatment of type 2 diabetes mellitus. To further facilitate structure-based drug discovery, we determined the crystal structure of rat CPT-2 (rCPT-2) in complex with the substrate analogue palmitoyl-aminocarnitine at 1.8A resolution. Biochemical analyses revealed a strong effect of this compound on rCPT-2 activity and stability. Using a computational approach we examined the membrane association of rCPT-2. The protein interacts with the membrane as a functional monomer and the calculations confirm the presence of a membrane association domain that consists of layers of hydrophobic and positively charged residues.


'''Crystal structure of rat carnitine palmitoyltransferase 2 in complex with r-3-(hexadecanoylamino)-4-(trimethylazaniumyl)butanoate'''
Carnitine palmitoyltransferase 2: analysis of membrane association and complex structure with a substrate analog.,Rufer AC, Lomize A, Benz J, Chomienne O, Thoma R, Hennig M FEBS Lett. 2007 Jul 10;581(17):3247-52. Epub 2007 Jun 8. PMID:17585909<ref>PMID:17585909</ref>


From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2rcu" style="background-color:#fffaf0;"></div>


==Overview==
==See Also==
The mitochondrial membrane-associated carnitine palmitoyltransferase system is a validated target for the treatment of type 2 diabetes mellitus. To further facilitate structure-based drug discovery, we determined the crystal structure of rat CPT-2 (rCPT-2) in complex with the substrate analogue palmitoyl-aminocarnitine at 1.8A resolution. Biochemical analyses revealed a strong effect of this compound on rCPT-2 activity and stability. Using a computational approach we examined the membrane association of rCPT-2. The protein interacts with the membrane as a functional monomer and the calculations confirm the presence of a membrane association domain that consists of layers of hydrophobic and positively charged residues.
*[[Carnitine palmitoyltransferase|Carnitine palmitoyltransferase]]
 
== References ==
==About this Structure==
<references/>
2RCU is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2RCU OCA].
__TOC__
 
</StructureSection>
==Reference==
[[Category: Large Structures]]
Carnitine palmitoyltransferase 2: analysis of membrane association and complex structure with a substrate analog., Rufer AC, Lomize A, Benz J, Chomienne O, Thoma R, Hennig M, FEBS Lett. 2007 Jul 10;581(17):3247-52. Epub 2007 Jun 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17585909 17585909]
[[Category: Carnitine O-palmitoyltransferase]]
[[Category: Rattus norvegicus]]
[[Category: Rattus norvegicus]]
[[Category: Single protein]]
[[Category: Benz J]]
[[Category: Benz, J.]]
[[Category: Chomienne O]]
[[Category: Chomienne, O.]]
[[Category: Hennig M]]
[[Category: Hennig, M.]]
[[Category: Rufer AC]]
[[Category: Rufer, A C.]]
[[Category: Thoma R]]
[[Category: Thoma, R.]]
[[Category: BOG]]
[[Category: BUJ]]
[[Category: acetylation]]
[[Category: acyltransferase]]
[[Category: fatty acid metabolism]]
[[Category: inner membrane]]
[[Category: lipid metabolism]]
[[Category: membrane]]
[[Category: mitochondrial protein]]
[[Category: mitochondrion]]
[[Category: transferase]]
[[Category: transferase 04-mai-06 r]]
[[Category: transit peptide]]
[[Category: transport]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:34:47 2008''

Latest revision as of 11:54, 30 August 2023

Crystal structure of rat carnitine palmitoyltransferase 2 in complex with r-3-(hexadecanoylamino)-4-(trimethylazaniumyl)butanoate

2rcu, resolution 1.78Å

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