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[[Image:2ref.jpg|left|200px]]


{{Structure
==Crystal structure of the loading GNATL domain of CurA from Lyngbya majuscula soaked with malonyl-CoA==
|PDB= 2ref |SIZE=350|CAPTION= <scene name='initialview01'>2ref</scene>, resolution 2.750&Aring;
<StructureSection load='2ref' size='340' side='right'caption='[[2ref]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
|SITE=
== Structural highlights ==
|LIGAND= <scene name='pdbligand=ACO:ACETYL COENZYME *A'>ACO</scene>
<table><tr><td colspan='2'>[[2ref]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorena_producens_19L Moorena producens 19L]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2REF FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
|GENE= curA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=158786 Lyngbya majuscula])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACO:ACETYL+COENZYME+*A'>ACO</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ref FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ref OCA], [https://pdbe.org/2ref PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ref RCSB], [https://www.ebi.ac.uk/pdbsum/2ref PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ref ProSAT]</span></td></tr>
 
</table>
'''Crystal structure of the loading GNATL domain of CurA from Lyngbya majuscula soaked with malonyl-CoA'''
== Function ==
 
[https://www.uniprot.org/uniprot/Q6DNF2_9CYAN Q6DNF2_9CYAN]  
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
An unexpected biochemical strategy for chain initiation is described for the loading module of the polyketide synthase of curacin A, an anticancer lead derived from the marine cyanobacterium Lyngbya majuscula. A central GCN5-related N-acetyltransferase (GNAT) domain bears bifunctional decarboxylase/S-acetyltransferase activity, both unprecedented for the GNAT superfamily. A CurA loading tridomain, consisting of an adaptor domain, the GNAT domain, and an acyl carrier protein, was assessed biochemically, revealing that a domain showing homology to GNAT (GNAT(L)) catalyzes (i) decarboxylation of malonyl-coenzyme A (malonyl-CoA) to acetyl-CoA and (ii) direct S-acetyl transfer from acetyl-CoA to load an adjacent acyl carrier protein domain (ACP(L)). Moreover, the N-terminal adapter domain was shown to facilitate acetyl-group transfer. Crystal structures of GNAT(L) were solved at 1.95 angstroms (ligand-free form) and 2.75 angstroms (acyl-CoA complex), showing distinct substrate tunnels for acyl-CoA and holo-ACP(L) binding. Modeling and site-directed mutagenesis experiments demonstrated that histidine-389 and threonine-355, at the convergence of the CoA and ACP tunnels, participate in malonyl-CoA decarboxylation but not in acetyl-group transfer. Decarboxylation precedes acetyl-group transfer, leading to acetyl-ACP(L) as the key curacin A starter unit.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/re/2ref_consurf.spt"</scriptWhenChecked>
2REF is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Lyngbya_majuscula Lyngbya majuscula]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2REF OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
GNAT-like strategy for polyketide chain initiation., Gu L, Geders TW, Wang B, Gerwick WH, Hakansson K, Smith JL, Sherman DH, Science. 2007 Nov 9;318(5852):970-4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/17991863 17991863]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ref ConSurf].
[[Category: Lyngbya majuscula]]
<div style="clear:both"></div>
[[Category: Single protein]]
__TOC__
[[Category: Geders, T W.]]
</StructureSection>
[[Category: Smith, J L.]]
[[Category: Large Structures]]
[[Category: ACO]]
[[Category: Moorena producens 19L]]
[[Category: curacin]]
[[Category: Geders TW]]
[[Category: decarboxylase]]
[[Category: Smith JL]]
[[Category: gnat]]
[[Category: loading]]
[[Category: lyase]]
[[Category: phosphopantetheine]]
[[Category: polyketide synthase]]
[[Category: s-acetyltransferase]]
[[Category: transferase]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:35:17 2008''
Retrieved from "https://proteopedia.org/2ref"