4z5v: Difference between revisions
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New page: '''Unreleased structure''' The entry 4z5v is ON HOLD Authors: Sui,B.K., Peng,G.Q., Zhao,L. Description: Crystal Structure of MHV ns2 PDE Domain Category: Unreleased Structures [[Ca... |
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==Crystal Structure of MHV ns2 PDE Domain== | |||
<StructureSection load='4z5v' size='340' side='right'caption='[[4z5v]], [[Resolution|resolution]] 3.05Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[4z5v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Murine_hepatitis_virus_strain_A59 Murine hepatitis virus strain A59]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4Z5V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4Z5V FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.049Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4z5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4z5v OCA], [https://pdbe.org/4z5v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4z5v RCSB], [https://www.ebi.ac.uk/pdbsum/4z5v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4z5v ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/NS2A_CVMA5 NS2A_CVMA5] Not essential for virus replication in transformed murine cells. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Prior studies have demonstrated that the mouse hepatitis virus A59 strain (MHV-A59) ns2 protein is a member of the 2H phosphoesterase family and exhibits 2',5'-phosphodiesterase (2',5'-PDE) activity. During the interferon antiviral response, ns2 cleaves 2',5'-oligoadenylate (2-5A), a key mediator of RNase L activation, thereby subverting the activation of RNase L and evading host innate immunity. However, the mechanism of 2-5A cleavage by ns2 remains unclear. Here, we present the crystal structure of the MHV ns2 PDE domain and demonstrate a PDE fold similar to that of the cellular protein, a kinase anchoring protein 7 central domain (AKAP7CD) and rotavirus VP3 carboxy-terminal domain (VP3-CTD). The structure displays a pair of strictly conserved HxS/Tx motifs and forms a deep positively charged catalytic groove with beta-sheets and an arginine containing loop (R-loop). These findings provide insight into the structural basis for 2-5A binding of MHV ns2. | |||
Crystal structure of the mouse hepatitis virus ns2 phosphodiesterase domain that antagonizes RNase L activation.,Sui B, Huang J, Jha BK, Yin P, Zhou M, Fu ZF, Silverman RH, Weiss SR, Peng G, Zhao L J Gen Virol. 2016 Jan 12. doi: 10.1099/jgv.0.000395. PMID:26757803<ref>PMID:26757803</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 4z5v" style="background-color:#fffaf0;"></div> | ||
[[Category: Peng | == References == | ||
[[Category: Sui | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Murine hepatitis virus strain A59]] | |||
[[Category: Huang JH]] | |||
[[Category: Peng GQ]] | |||
[[Category: Sui BK]] | |||
[[Category: Zhao L]] | |||
