1of5: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(20 intermediate revisions by the same user not shown)
Line 1: Line 1:
[[Image:1of5.gif|left|200px]]<br />
<applet load="1of5" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1of5, resolution 2.8&Aring;" />
'''CRYSTAL STRUCTURE OF MEX67-MTR2'''<br />


==Overview==
==Crystal structure of Mex67-Mtr2==
The association between Mtr2 and Mex67 is essential for the nuclear export, of bulk messenger RNA in yeast. In metazoans, the analogous function is, carried out by the TAP-p15 heterodimer. Whereas Mex67 and TAP are highly, conserved proteins, their binding partners, Mtr2 and p15, share no, sequence similarity, but are nevertheless functionally homologous. Here, we report the 2.8-A resolution crystal structure of Mtr2 in complex with, the NTF2-like domain of Mex67. Mtr2 is a novel member of the NTF2-like, family and interacts with Mex67, forming a complex with a similar, structural architecture to that of TAP-p15. Mtr2 fulfils an analogous, function to that of human p15 in maintaining the structural integrity of, the heterodimer. In addition, Mtr2 presents a long internal loop, which, contains residues that affect the export of the large ribosomal subunit.
<StructureSection load='1of5' size='340' side='right'caption='[[1of5]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1of5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OF5 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HG:MERCURY+(II)+ION'>HG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1of5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1of5 OCA], [https://pdbe.org/1of5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1of5 RCSB], [https://www.ebi.ac.uk/pdbsum/1of5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1of5 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/MEX67_YEAST MEX67_YEAST] Involved in the export of mRNA from the nucleus to the cytoplasm.<ref>PMID:9214641</ref>
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/of/1of5_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1of5 ConSurf].
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The association between Mtr2 and Mex67 is essential for the nuclear export of bulk messenger RNA in yeast. In metazoans, the analogous function is carried out by the TAP-p15 heterodimer. Whereas Mex67 and TAP are highly conserved proteins, their binding partners, Mtr2 and p15, share no sequence similarity, but are nevertheless functionally homologous. Here, we report the 2.8-A resolution crystal structure of Mtr2 in complex with the NTF2-like domain of Mex67. Mtr2 is a novel member of the NTF2-like family and interacts with Mex67, forming a complex with a similar structural architecture to that of TAP-p15. Mtr2 fulfils an analogous function to that of human p15 in maintaining the structural integrity of the heterodimer. In addition, Mtr2 presents a long internal loop, which contains residues that affect the export of the large ribosomal subunit.


==About this Structure==
Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15.,Fribourg S, Conti E EMBO Rep. 2003 Jul;4(7):699-703. PMID:12835756<ref>PMID:12835756</ref>
1OF5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae] with HG as [http://en.wikipedia.org/wiki/ligand ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1OF5 OCA].


==Reference==
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
Structural similarity in the absence of sequence homology of the messenger RNA export factors Mtr2 and p15., Fribourg S, Conti E, EMBO Rep. 2003 Jul;4(7):699-703. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12835756 12835756]
</div>
[[Category: Protein complex]]
<div class="pdbe-citations 1of5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Saccharomyces cerevisiae]]
[[Category: Conti, E.]]
[[Category: Conti E]]
[[Category: Fribourg, S.]]
[[Category: Fribourg S]]
[[Category: HG]]
[[Category: leucine-rich repeat]]
[[Category: mrna transport]]
[[Category: nuclear protein]]
[[Category: nuclear transport]]
[[Category: repeat]]
[[Category: transport]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 14:18:34 2007''
Retrieved from "https://proteopedia.org/1of5"