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==PaMorA tandem diguanylate cyclase - mutant phosphodiesterase, apo form==
==PaMorA tandem diguanylate cyclase - mutant phosphodiesterase, apo form==
<StructureSection load='4rnf' size='340' side='right' caption='[[4rnf]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
<StructureSection load='4rnf' size='340' side='right'caption='[[4rnf]], [[Resolution|resolution]] 2.85&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RNF FirstGlance]. <br>
<table><tr><td colspan='2'>[[4rnf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RNF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RNF FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4rnh|4rnh]], [[4rni|4rni]], [[4rnj|4rnj]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.85&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [http://www.ebi.ac.uk/pdbsum/4rnf PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rnf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rnf OCA], [https://pdbe.org/4rnf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rnf RCSB], [https://www.ebi.ac.uk/pdbsum/4rnf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rnf ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q9HVI8_PSEAE Q9HVI8_PSEAE]
Diguanylate cyclases (DGC) and phosphodiesterases (PDE), respectively synthesise and hydrolyse the secondary messenger cyclic dimeric GMP (c-di-GMP), and both activities are often found in a single protein. Intracellular c-di-GMP levels in turn regulate bacterial motility, virulence and biofilm formation. We report the first structure of a tandem DGC-PDE fragment, in which the catalytic domains are shown to be active. Two phosphodiesterase states are distinguished by active site formation. The structures, in the presence or absence of c-di-GMP, suggest that dimerisation and binding pocket formation are linked, with dimerisation being required for catalytic activity. An understanding of PDE activation is important, as biofilm dispersal via c-di-GMP hydrolysis has therapeutic effects on chronic infections.
 
Formation and dimerization of the phosphodiesterase active site of the Pseudomonas aeruginosa MorA, a bi-functional c-di-GMP regulator.,Phippen CW, Mikolajek H, Schlaefli HG, Keevil CW, Webb JS, Tews I FEBS Lett. 2014 Dec 20;588(24):4631-6. doi: 10.1016/j.febslet.2014.11.002. Epub, 2014 Nov 11. PMID:25447517<ref>PMID:25447517</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Phippen, C W]]
[[Category: Large Structures]]
[[Category: Tews, I]]
[[Category: Pseudomonas aeruginosa PAO1]]
[[Category: C-di-gmp]]
[[Category: Phippen CW]]
[[Category: Diguanylate cyclase]]
[[Category: Tews I]]
[[Category: Gtp]]
[[Category: Hydrolase]]
[[Category: Phosphodiesterase]]
[[Category: Tandem ggdef and eal domain]]
[[Category: Transferase]]

Latest revision as of 12:53, 1 March 2024

PaMorA tandem diguanylate cyclase - mutant phosphodiesterase, apo form

4rnf, resolution 2.85Å

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