4zbm: Difference between revisions

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'''Unreleased structure'''


The entry 4zbm is ON HOLD
==Crystal structure of Drosophila cyclic nucleotide gated channel pore mimicking NaK mutant==
<StructureSection load='4zbm' size='340' side='right'caption='[[4zbm]], [[Resolution|resolution]] 1.90&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[4zbm]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_14579 Bacillus cereus ATCC 14579]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ZBM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ZBM FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BA:BARIUM+ION'>BA</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4zbm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4zbm OCA], [https://pdbe.org/4zbm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4zbm RCSB], [https://www.ebi.ac.uk/pdbsum/4zbm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4zbm ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q81HW2_BACCR Q81HW2_BACCR]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Calcium permeability and the concomitant calcium block of monovalent ion current ("Ca2+ block") are properties of cyclic nucleotide-gated (CNG) channel fundamental to visual and olfactory signal transduction. Although most CNG channels bear a conserved glutamate residue crucial for Ca2+ block, the degree of block displayed by different CNG channels varies greatly. For instance, the Drosophila melanogaster CNG channel shows only weak Ca2+ block despite the presence of this glutamate. We previously constructed a series of chimeric channels in which we replaced the selectivity filter of the bacterial nonselective cation channel NaK with a set of CNG channel filter sequences and determined that the resulting NaK2CNG chimeras displayed the ion selectivity and Ca2+ block properties of the parent CNG channels. Here, we used the same strategy to determine the structural basis of the weak Ca2+ block observed in the Drosophila CNG channel. The selectivity filter of the Drosophila CNG channel is similar to that of most other CNG channels except that it has a threonine at residue 318 instead of a proline. We constructed a NaK chimera, which we called NaK2CNG-Dm, which contained the Drosophila selectivity filter sequence. The high resolution structure of NaK2CNG-Dm revealed a filter structure different from those of NaK and all other previously investigated NaK2CNG chimeric channels. Consistent with this structural difference, functional studies of the NaK2CNG-Dm chimeric channel demonstrated a loss of Ca2+ block compared with other NaK2CNG chimeras. Moreover, mutating the corresponding threonine (T318) to proline in Drosophila CNG channels increased Ca2+ block by 16 times. These results imply that a simple replacement of a threonine for a proline in Drosophila CNG channels has likely given rise to a distinct selectivity filter conformation that results in weak Ca2+ block.


Authors: Lam, Y., Zeng, W., Derebe, M.G., Jiang, Y.
Structural implications of weak Ca2+ block in Drosophila cyclic nucleotide-gated channels.,Lam YL, Zeng W, Derebe MG, Jiang Y J Gen Physiol. 2015 Aug 17. pii: jgp.201511431. PMID:26283200<ref>PMID:26283200</ref>


Description: Crystal structure of Drosophila cyclic nucleotide gated channel pore mimicking NaK mutant
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Jiang, Y]]
<div class="pdbe-citations 4zbm" style="background-color:#fffaf0;"></div>
[[Category: Lam, Y]]
== References ==
[[Category: Zeng, W]]
<references/>
[[Category: Derebe, M.G]]
__TOC__
</StructureSection>
[[Category: Bacillus cereus ATCC 14579]]
[[Category: Large Structures]]
[[Category: Derebe MG]]
[[Category: Jiang Y]]
[[Category: Lam Y]]
[[Category: Zeng W]]
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