Isopenicillin N synthase: Difference between revisions
From Proteopedia
Jump to navigationJump to search
Michal Harel (talk | contribs) No edit summary |
Michal Harel (talk | contribs) No edit summary |
||
| (9 intermediate revisions by 2 users not shown) | |||
| Line 1: | Line 1: | ||
<StructureSection load='1blz' size='350' side='right' scene='52/525170/Cv/1' caption='Isopenicilln N synthase complex with ACV and NO, [[1blz]]'> | |||
'''Isopenicillin N synthase''' (IPNS) is an iron-dependent enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV). IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics. The active site of IPNS contains an Fe atom. The reaction involves the reduction of O2 molecule to H2O. | == Function == | ||
'''Isopenicillin N synthase''' (IPNS) is an iron-dependent enzyme which catalyzes the formation of isopenicillin N (IPN) from the tripeptide aminoadipoyl-cysteine-valine (ACV). IPNS participates in the biosynthesis of penicillin and cephalosporin antibiotics. The active site of IPNS contains an Fe atom. The reaction involves the reduction of O2 molecule to H2O<ref>PMID:10537113</ref>. | |||
== Structural highlights == | |||
IPNS <scene name='52/525170/Cv/7'>active site contains Fe+2 and the substrate ACV</scene>. The <scene name='52/525170/Cv/8'>Fe+2 ion is hexacoordinated to 3 IPNS side chains, one water molecule, the ACV thiolate moiety and NO</scene><ref>PMID:9194566</ref>. Water molecules shown as red spheres. | |||
</StructureSection> | |||
==3D structures of isopenicillin N synthase== | ==3D structures of isopenicillin N synthase== | ||
Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}} | ||
{{#tree:id=OrganizedByTopic|openlevels=0| | {{#tree:id=OrganizedByTopic|openlevels=0| | ||
*IPNS | |||
**[[8bbb]] - AnIPNS + ACV – ''Aspergillus nidulans''<br /> | |||
**[[6jyv]] – IPNS – ''Pseudomonas aeruginosa''<br /> | |||
*Fe containing IPNS | *Fe containing IPNS | ||
**[[1bk0]] - AnIPNS + ACV | **[[1bk0]], [[6y0o]], [[6zae]], [[6zaf]], [[6zag]], [[6zah]], [[6zam]] - AnIPNS + ACV + Fe <br /> | ||
**[[2bjs]] - AnIPNS (mutant) + ACV<br /> | **[[2bjs]], [[7zoe]], [[8alj]], [[8bb9]], [[8bba]], [[8bbd]], [[8bsv]], [[8bsy]], [[8p47]], [[8ptv]] - AnIPNS (mutant) + ACV + Fe<br /> | ||
**[[ | **[[1hb1]], [[1hb2]], [[1hb3]], [[1hb4]], [[1oc1]], [[1odm]], [[1odn]], [[1qiq]], [[1qjf]], [[1uzw]], [[1w03]], [[1w05]], [[1w3v]], [[1w3x]], [[2bu9]], [[2ivi]], [[2ivj]], [[2vau]], [[2vbb]], [[2vbd]], [[2vbp]], [[2vcm]], [[2ve1]], [[2wo7]], [[2y60]], [[2y6f]], [[4bb3]], [[8p7p]], [[8py5]] - AnIPNS + ACV derivative + Fe<br /> | ||
**[[2jb4]] - AnIPNS + cyclobutanone analog<br /> | **[[8py8]], [[8py9]] - AnIPNS + ACV derivative + Fe + O2<br /> | ||
**[[2jb4]] - AnIPNS + cyclobutanone analog + Fe<br /> | |||
**[[6y0p]], [[6zao]], [[6zaq]], [[8py6]] - AnIPNS + IPN + Fe<br /> | |||
**[[8p7o]] - AnIPNS + inhibitor + Fe<br /> | |||
**[[7p3l]] - AnIPNS + substate analog + Fe<br /> | |||
**[[8p46]] - AnIPNS (mutant) + IPN + Fe<br /> | |||
**[[1qje]] - AnIPNS + IPN + ACV<br /> | **[[1qje]] - AnIPNS + IPN + ACV<br /> | ||
**[[1blz]] - AnIPNS + NO + ACV<br /> | **[[6zai]], [[6zaj]], [[6zal]], [[6zap]] - AnIPNS + O2 + ACV<br /> | ||
**[[7poy]] - AnIPNS (mutant) + NO + ACV<br /> | |||
**[[8py7]], [[8pya]] - AnIPNS (mutant) + Fe + O2 + ACV<br /> | |||
**[[7psw]], [[8ali]], [[8bbc]], [[8bsw]], [[8bsx]], [[8p48]], [[8rdb]] - AnIPNS (mutant) + Fe + ACV<br /> | |||
**[[1blz]], [[6zan]] - AnIPNS + NO + ACV<br /> | |||
**[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br /> | **[[1obn]], [[1w04]], [[1w06]] - AnIPNS + hydroxylamine + ACV derivative<br /> | ||
**[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br /> | **[[3zku]], [[3zky]], [[3zoi]], [[4bb3]] - IPNS + ACV derivative – ''Emericella nidulans''<br /> | ||
* | *Other metal containing IPNS | ||
**[[1ips]] – AnIPNS + Mn<br /> | |||
**[[6zw8]] - AnIPNS + ACV + Cd<br /> | |||
**[[8a4g]] - AnIPNS (mutant) + ACV + Cd<br /> | |||
**[[6zAK]] - AnIPNS + ACV + VO2<br /> | |||
}} | }} | ||
== References == | |||
<references/> | |||
[[Category: Topic Page]] | [[Category: Topic Page]] | ||
Latest revision as of 06:45, 8 October 2025
| ||||||||||||
3D structures of isopenicillin N synthase
Updated on 08-October-2025
