User:Wayne Decatur/Gal 4: Difference between revisions

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Gal4p is a transcriptional activator in ''Saccharomyces cerevisiae'' that regulates the expression of genes to coordinate the response to the carbon source galactose.
<StructureSection load='1d66' size='500' frame='true' side='right' caption='The N-terminal amino acids of Gal4p bound to DNA ([[1d66]])' scene='70/701975/1d66_firstglance/2' >
<StructureSection load='1d66' size='500' frame='true' side='right' caption='The N-terminal amino acids of Gal4p bound to DNA ([[1d66]])' scene='70/701975/1d66_firstglance/2' >
<!-- important to note you need to use 'side' in a StructureSEction instead of 'align'. If you leave 'align' it doesn't work.-->
<!-- important to note you need to use 'side' in a StructureSEction instead of 'align'. If you leave 'align' it doesn't work.-->
Gal4p is a transcriptional activator in ''Saccharomyces cerevisiae'' that regulates the expression of genes to coordinate the response to the carbon source galactose.
==Background==
==Background==
Gal4p is a transcriptional activator in ''Saccharomyces cerevisiae'' that regulates the expression of genes to coordinate the response to the carbon source galactose.
Gal4p is a transcriptional activator in ''Saccharomyces cerevisiae'' that regulates the expression of genes to coordinate the response to the carbon source galactose.
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The DNA binding domain <scene name='70/701975/1d66_firstglance_zn_binding/2'>uses six Cys residues to coordinate the binding of two ions of zinc (Zn(II))</scene>. (In the crystal structure [[1d66]], the zinc ions are actually represented by other members of the same group on the periodic table, the heavier cadmium ions.) The Zn-binding motif lets the structure accomplish more with fewer amino acids, the metals lending an "economy of structure", arranging two small helices to recognize a specific DNA site. <scene name='70/701975/1d66_base_conctacts/2'>Two lysines (17 and 18) make direct base contacts in the DNA major groove with the specified CGG triplets</scene>.<br>
The DNA binding domain <scene name='70/701975/1d66_firstglance_zn_binding/2'>uses six Cys residues to coordinate the binding of two ions of zinc (Zn(II))</scene>. (In the crystal structure [[1d66]], the zinc ions are actually represented by other members of the same group on the periodic table, the heavier cadmium ions.) The Zn-binding motif lets the structure accomplish more with fewer amino acids, the metals lending an "economy of structure", arranging two small helices to recognize a specific DNA site. <scene name='70/701975/1d66_base_conctacts/2'>Two lysines (17 and 18) make direct base contacts in the DNA major groove with the specified CGG triplets</scene>.<br>
{{Button Toggle NucleicDrumsColorSchemeWcartoon}} {{ColorKey Bases DNA}}
<jmol>
<jmolButton>
<script>select A,ADE,DA; color [x5050FF]; color cartoon [x5050FF]; select C, CYT,DC; color [xE00000]; color cartoon [xE00000]; select G,GUA,DG; color [x00C000]; color cartoon [x00C000]; select T,THY,DT; color [xE6E600]; color cartoon [xE6E600]; select U,URA; color [xCC9900]; color cartoon [xCC9900];</script>
<text>Color Structure by Nucleotide</text>
</jmolButton>
</jmol>
{{ColorKey Bases DNA}}


Despite only making base-specific contacts at the ends, <scene name='70/701975/Spacefill/1'>Ga14p is mostly in contact with the DNA for its entire span of the binding site.</scene>
Despite only making base-specific contacts at the ends, <scene name='70/701975/Spacefill/1'>Ga14p is mostly in contact with the DNA for its entire span of the binding site.</scene>
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