2z8o: Difference between revisions

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[[Image:2z8o.jpg|left|200px]]


{{Structure
==Structural basis for the catalytic mechanism of phosphothreonine lyase==
|PDB= 2z8o |SIZE=350|CAPTION= <scene name='initialview01'>2z8o</scene>, resolution 2.4&Aring;
<StructureSection load='2z8o' size='340' side='right'caption='[[2z8o]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=TLA:L(+)-TARTARIC ACID'>TLA</scene>
<table><tr><td colspan='2'>[[2z8o]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2Z8O FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
|GENE= mkaD, spvC, vsdD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=TLA:L(+)-TARTARIC+ACID'>TLA</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2z8o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8o OCA], [https://pdbe.org/2z8o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2z8o RCSB], [https://www.ebi.ac.uk/pdbsum/2z8o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2z8o ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/SPVC_SALTY SPVC_SALTY] Secreted effector that irreversibly inactivates host MAP kinases by catalyzing the dephosphorylation of the phosphothreonine residue in the pT-X-pY motif in MAPK2/ERK2, MAPK3/ERK1, and p38, via a beta-elimination reaction leading to a dehydrobutyrine residue. Is also able to remove the phosphate group from phospho-JNK in vitro, but JNK may not be a substrate in vivo. Could help suppress localized proinflammatory responses at infection foci in the spleen and liver, and thereby facilitate bacterial growth.<ref>PMID:17303758</ref> <ref>PMID:18060821</ref> <ref>PMID:18084305</ref> <ref>PMID:18284579</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Salmonella SpvC belongs to a new enzyme family designated phosphothreonine lyases that irreversibly inactivate mitogen-activated protein kinases. The crystal structure of SpvC reported here reveals that the two phosphorylated residues in the substrate peptide predominantly mediate its recognition by SpvC. Substrate-induced conformational changes in SpvC sequester the phosphothreonine in a completely solvent-free environment, preventing the hydrolysis of the phosphate group and facilitating the elimination reaction.


'''Structural basis for the catalytic mechanism of phosphothreonine lyase'''
Structural basis for the catalytic mechanism of phosphothreonine lyase.,Chen L, Wang H, Zhang J, Gu L, Huang N, Zhou JM, Chai J Nat Struct Mol Biol. 2008 Jan;15(1):101-2. Epub 2007 Dec 16. PMID:18084305<ref>PMID:18084305</ref>


 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
==About this Structure==
</div>
2Z8O is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhimurium Salmonella typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2Z8O OCA].
<div class="pdbe-citations 2z8o" style="background-color:#fffaf0;"></div>
[[Category: Salmonella typhimurium]]
== References ==
[[Category: Single protein]]
<references/>
[[Category: Chai, J J.]]
__TOC__
[[Category: Chen, L J.]]
</StructureSection>
[[Category: Gu, L C.]]
[[Category: Large Structures]]
[[Category: Huang, N.]]
[[Category: Salmonella enterica subsp. enterica serovar Typhimurium]]
[[Category: Wang, H Y.]]
[[Category: Chai J]]
[[Category: Zhou, J M.]]
[[Category: Chen L]]
[[Category: TLA]]
[[Category: Gu L]]
[[Category: distorted beta-strand sheet]]
[[Category: Huang N]]
[[Category: lyase]]
[[Category: Wang H]]
[[Category: short three-helix bundle]]
[[Category: Zhou JM]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:52:55 2008''
Retrieved from "https://proteopedia.org/2z8o"