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[[Image:2zb9.jpg|left|200px]]


{{Structure
==Crystal structure of TetR family transcription regulator SCO0332==
|PDB= 2zb9 |SIZE=350|CAPTION= <scene name='initialview01'>2zb9</scene>, resolution 2.25&Aring;
<StructureSection load='2zb9' size='340' side='right'caption='[[2zb9]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[2zb9]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZB9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2ZB9 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25&#8491;</td></tr>
|GENE= sco0332 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1902 Streptomyces coelicolor])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2zb9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zb9 OCA], [https://pdbe.org/2zb9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2zb9 RCSB], [https://www.ebi.ac.uk/pdbsum/2zb9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2zb9 ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''Crystal structure of TetR family transcription regulator SCO0332'''
[https://www.uniprot.org/uniprot/Q9RK47_STRCO Q9RK47_STRCO]
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
SCO0332 protein is a putative TetR-family transcriptional regulator from Streptomyces coelicolor A3(2). The crystal structure of SCO0332 was determined at 2.25 A resolution by single-wavelength anomalous diffraction (SAD) phasing using the S atoms of the native protein. SCO0332 contains a helix-turn-helix (HTH) DNA-binding motif in its N-terminal region and forms a homodimer. The overall structure of SCO0332 shows significant similarity to other TetR-family regulators. A systematic evolution of ligands by exponential enrichment (SELEX) analysis indicated that SCO0332 has sequence-specific DNA-binding ability and determined the position of the operator element of SCO0332 on the chromosomal DNA of S. coelicolor. An electrophoretic mobility-shift assay (EMSA) showed that SCO0332 binds to the operator sequence upstream of the sco0330 gene, which encodes a putative short-chain oxidoreductase. These results suggest that SCO0332 is a transcriptional repressor that regulates sco0330 gene expression.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/2zb9_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
2ZB9 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_coelicolor Streptomyces coelicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZB9 OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2zb9 ConSurf].
Structural and functional analysis of the TetR-family transcriptional regulator SCO0332 from Streptomyces coelicolor., Okada U, Kondo K, Hayashi T, Watanabe N, Yao M, Tamura T, Tanaka I, Acta Crystallogr D Biol Crystallogr. 2008 Feb;64(Pt 2):198-205. Epub 2008, Jan 16. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18219120 18219120]
<div style="clear:both"></div>
[[Category: Single protein]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Streptomyces coelicolor]]
[[Category: Streptomyces coelicolor]]
[[Category: Kondo, K.]]
[[Category: Kondo K]]
[[Category: Okada, U.]]
[[Category: Okada U]]
[[Category: Tanaka, I.]]
[[Category: Tanaka I]]
[[Category: Watanabe, N.]]
[[Category: Watanabe N]]
[[Category: Yao, M.]]
[[Category: Yao M]]
[[Category: dna-binding]]
[[Category: helix-turn-helix]]
[[Category: tetr family]]
[[Category: transcription regulation]]
[[Category: transcription regulator]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:53:32 2008''

Latest revision as of 13:56, 13 March 2024

Crystal structure of TetR family transcription regulator SCO0332

2zb9, resolution 2.25Å

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