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[[Image:379d.gif|left|200px]]


{{Structure
==THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE==
|PDB= 379d |SIZE=350|CAPTION= <scene name='initialview01'>379d</scene>, resolution 3.100&Aring;
<StructureSection load='379d' size='340' side='right'caption='[[379d]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=CO:COBALT (II) ION'>CO</scene>
<table><tr><td colspan='2'>[[379d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=379D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=379D FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=379d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=379d OCA], [https://pdbe.org/379d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=379d RCSB], [https://www.ebi.ac.uk/pdbsum/379d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=379d ProSAT]</span></td></tr>
</table>


'''THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE'''
==See Also==
 
*[[Ribozyme|Ribozyme]]
 
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
==Overview==
__TOC__
We have captured an 8.7 A conformational change that takes place in the cleavage site of the hammerhead ribozyme during self-cleavage, using X-ray crystallography combined with physical and chemical trapping techniques. This rearrangement brings the hammerhead ribozyme from the ground state into a conformation that is poised to form the transition state geometry required for hammerhead RNA self-cleavage. Use of a 5'-C-methylated ribose adjacent to the cleavage site permits this ordinarily transient conformational change to be kinetically trapped and observed crystallographically after initiating the hammerhead ribozyme reaction in the crystal. Cleavage of the corresponding unmodified hammerhead ribozyme in the crystal under otherwise identical conditions is faster than in solution, indicating that we have indeed trapped a catalytically relevant intermediate form of this RNA enzyme.
</StructureSection>
 
[[Category: Large Structures]]
==About this Structure==
[[Category: Beigelman L]]
379D is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=379D OCA].
[[Category: Karpeisky A]]
 
[[Category: Maloney L]]
==Reference==
[[Category: Murray JB]]
The structural basis of hammerhead ribozyme self-cleavage., Murray JB, Terwey DP, Maloney L, Karpeisky A, Usman N, Beigelman L, Scott WG, Cell. 1998 Mar 6;92(5):665-73. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9506521 9506521]
[[Category: Scott WG]]
[[Category: Protein complex]]
[[Category: Terwey DP]]
[[Category: Beigelman, L.]]
[[Category: Usman N]]
[[Category: Karpeisky, A.]]
[[Category: Maloney, L.]]
[[Category: Murray, J B.]]
[[Category: Scott, W G.]]
[[Category: Terwey, D P.]]
[[Category: Usman, N.]]
[[Category: CO]]
[[Category: catalytic rna]]
[[Category: loop]]
[[Category: rna hammerhead ribozyme]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:55:07 2008''

Latest revision as of 08:39, 7 February 2024

THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE

379d, resolution 3.10Å

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