3bf0: Difference between revisions

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[[Image:3bf0.jpg|left|200px]]


{{Structure
==Crystal structure of Escherichia coli Signal peptide peptidase (SppA), Native crystals==
|PDB= 3bf0 |SIZE=350|CAPTION= <scene name='initialview01'>3bf0</scene>, resolution 2.550&Aring;
<StructureSection load='3bf0' size='340' side='right'caption='[[3bf0]], [[Resolution|resolution]] 2.55&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND=  
<table><tr><td colspan='2'>[[3bf0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BF0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BF0 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.55&#8491;</td></tr>
|GENE= sppA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bf0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bf0 OCA], [https://pdbe.org/3bf0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bf0 RCSB], [https://www.ebi.ac.uk/pdbsum/3bf0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bf0 ProSAT]</span></td></tr>
}}
</table>
 
== Function ==
'''Crystal structure of Escherichia coli Signal peptide peptidase (SppA), Native crystals'''
[https://www.uniprot.org/uniprot/SPPA_ECOLI SPPA_ECOLI] Digests cleaved signal peptides in vitro, its in vivo function is unknown. This activity is necessary to maintain proper secretion of mature proteins across the membrane.<ref>PMID:3522590</ref> <ref>PMID:18476724</ref> <ref>PMID:21810987</ref> <ref>PMID:18164727</ref>
 
== Evolutionary Conservation ==
 
[[Image:Consurf_key_small.gif|200px|right]]
==Overview==
Check<jmol>
Signal peptide peptidase (Spp) is the enzyme responsible for cleaving the remnant signal peptides left behind in the membrane following Sec-dependent protein secretion. Spp activity appears to be present in all cell types, eukaryotic, prokaryotic and archaeal. Here we report the first structure of a signal peptide peptidase, that of the Escherichia coli SppA (SppA(EC)). SppA(EC) forms a tetrameric assembly with a novel bowl-shaped architecture. The bowl has a dramatically hydrophobic interior and contains four separate active sites that utilize a Ser/Lys catalytic dyad mechanism. Our structural analysis of SppA reveals that while in many Gram-negative bacteria as well as characterized plant variants, a tandem duplication in the protein fold creates an intact active site at the interface between the repeated domains, other species, particularly Gram-positive and archaeal organisms, encode half-size, unduplicated SppA variants that could form similar oligomers to their duplicated counterparts, but using an octamer arrangement and with the catalytic residues provided by neighboring monomers. The structure reveals a similarity in the protein fold between the domains in the periplasmic Ser/Lys protease SppA and the monomers seen in the cytoplasmic Ser/His/Asp protease ClpP. We propose that SppA may, in addition to its role in signal peptide hydrolysis, have a role in the quality assurance of periplasmic and membrane-bound proteins, similar to the role that ClpP plays for cytoplasmic proteins.
  <jmolCheckbox>
 
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bf/3bf0_consurf.spt"</scriptWhenChecked>
==About this Structure==
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
3BF0 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BF0 OCA].  
    <text>to colour the structure by Evolutionary Conservation</text>
 
  </jmolCheckbox>
==Reference==
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bf0 ConSurf].
Crystal structure of a bacterial signal Peptide peptidase., Kim AC, Oliver DC, Paetzel M, J Mol Biol. 2008 Feb 15;376(2):352-66. Epub 2007 Dec 4. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18164727 18164727]
<div style="clear:both"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Paetzel, M.]]
[[Category: Paetzel M]]
[[Category: bacterial]]
[[Category: hydrolase]]
[[Category: inner membrane]]
[[Category: membrane]]
[[Category: protease]]
[[Category: ser/lys protease]]
[[Category: transmembrane]]
 
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Retrieved from "https://proteopedia.org/3bf0"