2n3d: Difference between revisions
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The | ==Atomic structure of the cytoskeletal bactofilin BacA revealed by solid-state NMR== | ||
<StructureSection load='2n3d' size='340' side='right'caption='[[2n3d]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[2n3d]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Caulobacter_vibrioides_CB15 Caulobacter vibrioides CB15]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2N3D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2N3D FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solid-state NMR</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2n3d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2n3d OCA], [https://pdbe.org/2n3d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2n3d RCSB], [https://www.ebi.ac.uk/pdbsum/2n3d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2n3d ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9A753_CAUVC Q9A753_CAUVC] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Bactofilins are a recently discovered class of cytoskeletal proteins of which no atomic-resolution structure has been reported thus far. The bacterial cytoskeleton plays an essential role in a wide range of processes, including morphogenesis, cell division, and motility. Among the cytoskeletal proteins, the bactofilins are bacteria-specific and do not have a eukaryotic counterpart. The bactofilin BacA of the species Caulobacter crescentus is not amenable to study by x-ray crystallography or solution nuclear magnetic resonance (NMR) because of its inherent noncrystallinity and insolubility. We present the atomic structure of BacA calculated from solid-state NMR-derived distance restraints. We show that the core domain of BacA forms a right-handed beta helix with six windings and a triangular hydrophobic core. The BacA structure was determined to 1.0 A precision (heavy-atom root mean square deviation) on the basis of unambiguous restraints derived from four-dimensional (4D) HN-HN and 2D C-C NMR spectra. | |||
Atomic-resolution structure of cytoskeletal bactofilin by solid-state NMR.,Shi C, Fricke P, Lin L, Chevelkov V, Wegstroth M, Giller K, Becker S, Thanbichler M, Lange A Sci Adv. 2015 Dec 4;1(11):e1501087. doi: 10.1126/sciadv.1501087. eCollection 2015, Dec. PMID:26665178<ref>PMID:26665178</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Becker | <div class="pdbe-citations 2n3d" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Caulobacter vibrioides CB15]] | ||
[[Category: Shi | [[Category: Large Structures]] | ||
[[Category: Wegstroth | [[Category: Becker S]] | ||
[[Category: Chevelkov V]] | |||
[[Category: Fricke P]] | |||
[[Category: Giller K]] | |||
[[Category: Lange A]] | |||
[[Category: Lin L]] | |||
[[Category: Shi C]] | |||
[[Category: Thanbichler M]] | |||
[[Category: Wegstroth M]] | |||
