5bu8: Difference between revisions

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New page: '''Unreleased structure''' The entry 5bu8 is ON HOLD Authors: Sankhala, R.S., Cingolani, G. Description: HK620 Tail Needle crystallized at pH 7.5 and derivatized with Xenon [[Category:...
 
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'''Unreleased structure'''


The entry 5bu8 is ON HOLD
==HK620 Tail Needle crystallized at pH 7.5 and derivatized with Xenon==
<StructureSection load='5bu8' size='340' side='right'caption='[[5bu8]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5bu8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_HK620 Enterobacteria phage HK620]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5BU8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5BU8 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.202&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=XE:XENON'>XE</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5bu8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5bu8 OCA], [https://pdbe.org/5bu8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5bu8 RCSB], [https://www.ebi.ac.uk/pdbsum/5bu8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5bu8 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q9AYZ3_BPHK6 Q9AYZ3_BPHK6]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Bacterial viruses of the P22-like family encode a specialized tail needle essential for genome stabilization after DNA-packaging and implicated in Gram-negative cell envelope penetration. The atomic structure of P22 tail needle (gp26) crystallized at acidic pH reveals a slender fiber containing an N-terminal trimer-of-hairpins tip. Though the length and composition of tail needles vary significantly in Podoviridae, unexpectedly, the amino acid sequence of the N-terminal tip is exceptionally conserved in more than two hundred genomes of P22-like phages and prophages. In this paper, we used X-ray crystallography and EM to investigate the neutral pH structure of three tail needles from bacteriophage P22, HK620 and Sf6. In all cases, we found the N-terminal tip is poorly structured, in stark contrast to the compact trimer-of-hairpins seen in gp26 crystallized at acidic pH. Hydrogen/deuterium exchange mass spectrometry, limited proteolysis, circular dichroism spectroscopy and gel filtration chromatography revealed that the N-terminal tip is highly dynamic in solution and unlikely to adopt a stable trimeric conformation at physiological pH. This is supported by the cryo-EM reconstruction of P22 mature virion tail, where the density of gp26 N-terminal tip is incompatible with a trimer-of-hairpins. We propose the tail needle N-terminal tip exists in two conformations: a pre-ejection extended conformation, which seals the portal vertex after genome-packaging and a post-ejection trimer-of-hairpins that form upon its release from the virion. The conformational plasticity of the tail needle N-terminal tip is built in the amino acid sequence, explaining its extraordinary conservation in nature.


Authors: Sankhala, R.S., Cingolani, G.
Structural plasticity of the protein plug that traps newly packaged genomes in podoviridae virions.,Bhardwaj A, Sankhala RS, Olia AS, Brooke D, Casjens SR, Taylor DJ, Prevelige PE Jr, Cingolani G J Biol Chem. 2015 Nov 16. pii: jbc.M115.696260. PMID:26574546<ref>PMID:26574546</ref>


Description: HK620 Tail Needle crystallized at pH 7.5 and derivatized with Xenon
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Sankhala, R.S]]
<div class="pdbe-citations 5bu8" style="background-color:#fffaf0;"></div>
[[Category: Cingolani, G]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Enterobacteria phage HK620]]
[[Category: Large Structures]]
[[Category: Cingolani G]]
[[Category: Sankhala RS]]
Retrieved from "https://proteopedia.org/5bu8"