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| [[Image:3bk6.jpg|left|200px]]
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| {{Structure
| | ==Crystal structure of a core domain of stomatin from Pyrococcus horikoshii== |
| |PDB= 3bk6 |SIZE=350|CAPTION= <scene name='initialview01'>3bk6</scene>, resolution 3.20Å
| | <StructureSection load='3bk6' size='340' side='right'caption='[[3bk6]], [[Resolution|resolution]] 3.20Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[3bk6]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BK6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3BK6 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| |GENE= PH1511 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3bk6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3bk6 OCA], [https://pdbe.org/3bk6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3bk6 RCSB], [https://www.ebi.ac.uk/pdbsum/3bk6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3bk6 ProSAT]</span></td></tr> |
| }}
| | </table> |
| | | == Function == |
| '''Crystal structure of a core domain of stomatin from Pyrococcus horikoshii''' | | [https://www.uniprot.org/uniprot/PSTOM_PYRHO PSTOM_PYRHO] |
| | | == Evolutionary Conservation == |
| | | [[Image:Consurf_key_small.gif|200px|right]] |
| ==Overview== | | Check<jmol> |
| Stomatin is a major integral membrane protein of human erythrocytes, the absence of which is associated with a form of hemolytic anemia known as hereditary stomatocytosis. However, the function of stomatin is not fully understood. An open reading frame, PH1511, from the hyperthermophilic archaeon Pyrococcus horikoshii encodes p-stomatin, a prokaryotic stomatin. Here, we report the first crystal structure of a stomatin ortholog, the core domain of the p-stomatin PH1511p (residues 56-234 of PH1511p, designated as PhSto(CD)). PhSto(CD) forms a novel homotrimeric structure. Three alpha/beta domains form a triangle of about 50 A on each side, and three alpha-helical segments of about 60 A in length extend from the apexes of the triangle. The alpha/beta domain of PhSto(CD) is partly similar in structure to the band-7 domain of mouse flotillin-2. While the alpha/beta domain is relatively rigid, the alpha-helical segment shows conformational flexibility, adapting to the neighboring environment. One alpha-helical segment forms an anti-parallel coiled coil with another alpha-helical segment from a symmetry-related molecule. The alpha-helical segment shows a heptad repeat pattern, and mainly hydrophobic residues form a coiled-coil interface. According to chemical cross-linking experiments, PhSto(CD) would be able to assemble into an oligomeric form. The coiled-coil fold observed in the crystal probably contributes to self-association.
| | <jmolCheckbox> |
| | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bk/3bk6_consurf.spt"</scriptWhenChecked> |
| ==About this Structure== | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| 3BK6 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3BK6 OCA].
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | | </jmolCheckbox> |
| ==Reference==
| | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=3bk6 ConSurf]. |
| Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold., Yokoyama H, Fujii S, Matsui I, J Mol Biol. 2008 Feb 22;376(3):868-78. Epub 2008 Jan 8. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18182167 18182167]
| | <div style="clear:both"></div> |
| | __TOC__ |
| | </StructureSection> |
| | [[Category: Large Structures]] |
| [[Category: Pyrococcus horikoshii]] | | [[Category: Pyrococcus horikoshii]] |
| [[Category: Single protein]]
| | [[Category: Fujii S]] |
| [[Category: Fujii, S.]] | | [[Category: Matsui I]] |
| [[Category: Matsui, I.]] | | [[Category: Yokoyama H]] |
| [[Category: Yokoyama, H.]] | |
| [[Category: archaea]]
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| [[Category: coiled-coil]]
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| [[Category: flotillin]]
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| [[Category: membrane fusion]]
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| [[Category: membrane protein]]
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| [[Category: pyrococcus horikoshii]]
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| [[Category: spfh]]
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| [[Category: stomatin]]
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| [[Category: trafficking]]
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| [[Category: transmembrane]]
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| [[Category: trimer]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 18:59:44 2008''
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