5c4i: Difference between revisions

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'''Unreleased structure'''


The entry 5c4i is ON HOLD
==Structure of an Oxalate Oxidoreductase==
<StructureSection load='5c4i' size='340' side='right'caption='[[5c4i]], [[Resolution|resolution]] 2.27&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5c4i]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Moorella_thermoacetica_ATCC_39073 Moorella thermoacetica ATCC 39073]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C4I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C4I FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.274&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TPP:THIAMINE+DIPHOSPHATE'>TPP</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c4i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c4i OCA], [https://pdbe.org/5c4i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c4i RCSB], [https://www.ebi.ac.uk/pdbsum/5c4i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c4i ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/OORA_MOOTA OORA_MOOTA] Catalyzes the anaerobic oxidation of oxalate using a broad range of electron acceptors, including ferredoxin and the nickel-dependent carbon monoxide dehydrogenase. Does not require coenzyme A as cosubstrate. Enables anaerobic growth on oxalate which is used as energy source by the bacteria.<ref>PMID:20956531</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Thiamine pyrophosphate (TPP), a derivative of vitamin B1, is a versatile and ubiquitous cofactor. When coupled with [4Fe-4S] clusters in microbial 2-oxoacid:ferredoxin oxidoreductases (OFORs), TPP is involved in catalyzing low-potential redox reactions that are important for the synthesis of key metabolites and the reduction of N2, H+, and CO2. We have determined the high-resolution (2.27 A) crystal structure of the TPP-dependent oxalate oxidoreductase (OOR), an enzyme that allows microbes to grow on oxalate, a widely occurring dicarboxylic acid that is found in soil and freshwater and is responsible for kidney stone disease in humans. OOR catalyzes the anaerobic oxidation of oxalate, harvesting the low-potential electrons for use in anaerobic reduction and fixation of CO2. We compare the OOR structure to that of the only other structurally characterized OFOR family member, pyruvate:ferredoxin oxidoreductase. This side-by-side structural analysis highlights the key similarities and differences that are relevant for the chemistry of this entire class of TPP-utilizing enzymes.


Authors: Gibson, Marcus I., Brignole, Edward J., Pierce, Elizabeth, Can, Mehmet, Ragsdale, Stephen W., Drennan, Catherine L.
The Structure of an Oxalate Oxidoreductase Provides Insight into Microbial 2-Oxoacid Metabolism.,Gibson MI, Brignole EJ, Pierce E, Can M, Ragsdale SW, Drennan CL Biochemistry. 2015 Jun 24. PMID:26061898<ref>PMID:26061898</ref>


Description: Structure of an Oxalate Oxidoreductase
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Pierce, Elizabeth, Can, Mehmet, Ragsdale, Stephen W]]
<div class="pdbe-citations 5c4i" style="background-color:#fffaf0;"></div>
[[Category: Brignole, Edward J]]
== References ==
[[Category: Drennan, Catherine L]]
<references/>
[[Category: Gibson, Marcus I]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Moorella thermoacetica ATCC 39073]]
[[Category: Brignole EJ]]
[[Category: Can M]]
[[Category: Drennan CL]]
[[Category: Gibson MI]]
[[Category: Pierce E]]
[[Category: Ragsdale SW]]
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