5c6g: Difference between revisions
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New page: '''Unreleased structure''' The entry 5c6g is ON HOLD until Paper Publication Authors: Singleton, M.R., Chao, W.C.H. Description: Category: Unreleased Structures [[Category: Chao, ... |
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==Structural Insights into the Scc2-Scc4 Cohesin Loader== | |||
<StructureSection load='5c6g' size='340' side='right'caption='[[5c6g]], [[Resolution|resolution]] 2.60Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5c6g]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Eremothecium_gossypii_ATCC_10895 Eremothecium gossypii ATCC 10895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C6G FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6g OCA], [https://pdbe.org/5c6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c6g RCSB], [https://www.ebi.ac.uk/pdbsum/5c6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6g ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q74ZR5_ASHGO Q74ZR5_ASHGO] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The remarkable accuracy of eukaryotic cell division is partly maintained by the cohesin complex acting as a molecular glue to prevent premature sister chromatid separation. The loading of cohesin onto chromosomes is catalyzed by the Scc2-Scc4 loader complex. Here, we report the crystal structure of Scc4 bound to the N terminus of Scc2 and show that Scc4 is a tetratricopeptide repeat (TPR) superhelix. The Scc2 N terminus adopts an extended conformation and is entrapped by the core of the Scc4 superhelix. Electron microscopy (EM) analysis reveals that the Scc2-Scc4 loader complex comprises three domains: a head, body, and hook. Deletion studies unambiguously assign the Scc2N-Scc4 as the globular head domain, whereas in vitro cohesin loading assays show that the central body and the hook domains are sufficient to catalyze cohesin loading onto circular DNA, but not chromatinized DNA in vivo, suggesting a possible role for Scc4 as a chromatin adaptor. | |||
Structural Studies Reveal the Functional Modularity of the Scc2-Scc4 Cohesin Loader.,Chao WC, Murayama Y, Munoz S, Costa A, Uhlmann F, Singleton MR Cell Rep. 2015 Jul 22. pii: S2211-1247(15)00709-3. doi:, 10.1016/j.celrep.2015.06.071. PMID:26212329<ref>PMID:26212329</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Chao | <div class="pdbe-citations 5c6g" style="background-color:#fffaf0;"></div> | ||
[[Category: Singleton | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Eremothecium gossypii ATCC 10895]] | |||
[[Category: Large Structures]] | |||
[[Category: Chao WCH]] | |||
[[Category: Singleton MR]] | |||
Latest revision as of 11:44, 9 May 2024
Structural Insights into the Scc2-Scc4 Cohesin Loader
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