5c6m: Difference between revisions
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New page: '''Unreleased structure''' The entry 5c6m is ON HOLD until Paper Publication Authors: Weiergraeber, O.H., Dick, M., Bramski, J., Pietruszka, J. Description: Crystal structure of deoxyr... |
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==Crystal structure of deoxyribose-phosphate aldolase from Shewanella halifaxensis== | |||
<StructureSection load='5c6m' size='340' side='right'caption='[[5c6m]], [[Resolution|resolution]] 1.76Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5c6m]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Shewanella_halifaxensis Shewanella halifaxensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5C6M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5C6M FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.76Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5c6m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5c6m OCA], [https://pdbe.org/5c6m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5c6m RCSB], [https://www.ebi.ac.uk/pdbsum/5c6m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5c6m ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/DEOC_SHEHH DEOC_SHEHH] Catalyzes a reversible aldol reaction between acetaldehyde and D-glyceraldehyde 3-phosphate to generate 2-deoxy-D-ribose 5-phosphate. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Understanding enzyme stability and activity in extremophilic organisms is of great biotechnological interest, but many questions are still unsolved. Using 2-deoxy-D-ribose-5-phosphate aldolase (DERA) as model enzyme, we have evaluated structural and functional characteristics of different orthologs from psychrophilic, mesophilic and hyperthermophilic organisms. We present the first crystal structures of psychrophilic DERAs, revealing a dimeric organization resembling their mesophilic but not their thermophilic counterparts. Conversion into monomeric proteins showed that the native dimer interface contributes to stability only in the hyperthermophilic enzymes. Nevertheless, introduction of a disulfide bridge in the interface of a psychrophilic DERA did confer increased thermostability, suggesting a strategy for rational design of more durable enzyme variants. Constraint network analysis revealed particularly sparse interactions between the substrate pocket and its surrounding alpha-helices in psychrophilic DERAs, which indicates that a more flexible active center underlies their high turnover numbers. | |||
Trading off stability against activity in extremophilic aldolases.,Dick M, Weiergraber OH, Classen T, Bisterfeld C, Bramski J, Gohlke H, Pietruszka J Sci Rep. 2016 Jan 19;6:17908. doi: 10.1038/srep17908. PMID:26783049<ref>PMID:26783049</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Bramski | <div class="pdbe-citations 5c6m" style="background-color:#fffaf0;"></div> | ||
[[Category: | |||
[[Category: Pietruszka | ==See Also== | ||
[[Category: | *[[Aldolase 3D structures|Aldolase 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Shewanella halifaxensis]] | |||
[[Category: Bramski J]] | |||
[[Category: Dick M]] | |||
[[Category: Pietruszka J]] | |||
[[Category: Weiergraeber OH]] | |||
Latest revision as of 11:18, 10 January 2024
Crystal structure of deoxyribose-phosphate aldolase from Shewanella halifaxensis
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