5a5u: Difference between revisions
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==Structure of mammalian eIF3 in the context of the 43S preinitiation complex== | |||
<SX load='5a5u' size='340' side='right' viewer='molstar' caption='[[5a5u]], [[Resolution|resolution]] 9.00Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5a5u]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Oryctolagus_cuniculus Oryctolagus cuniculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A5U OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A5U FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 9Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a5u FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a5u OCA], [https://pdbe.org/5a5u PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a5u RCSB], [https://www.ebi.ac.uk/pdbsum/5a5u PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a5u ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G1SZ03_RABIT G1SZ03_RABIT] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
During eukaryotic translation initiation, 43S complexes, comprising a 40S ribosomal subunit, initiator transfer RNA and initiation factors (eIF) 2, 3, 1 and 1A, attach to the 5'-terminal region of messenger RNA and scan along it to the initiation codon. Scanning on structured mRNAs also requires the DExH-box protein DHX29. Mammalian eIF3 contains 13 subunits and participates in nearly all steps of translation initiation. Eight subunits having PCI (proteasome, COP9 signalosome, eIF3) or MPN (Mpr1, Pad1, amino-terminal) domains constitute the structural core of eIF3, to which five peripheral subunits are flexibly linked. Here we present a cryo-electron microscopy structure of eIF3 in the context of the DHX29-bound 43S complex, showing the PCI/MPN core at approximately 6 A resolution. It reveals the organization of the individual subunits and their interactions with components of the 43S complex. We were able to build near-complete polyalanine-level models of the eIF3 PCI/MPN core and of two peripheral subunits. The implications for understanding mRNA ribosomal attachment and scanning are discussed. | |||
Structure of mammalian eIF3 in the context of the 43S preinitiation complex.,des Georges A, Dhote V, Kuhn L, Hellen CU, Pestova TV, Frank J, Hashem Y Nature. 2015 Sep 24;525(7570):491-5. doi: 10.1038/nature14891. Epub 2015 Sep 7. PMID:26344199<ref>PMID:26344199</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5a5u" style="background-color:#fffaf0;"></div> | ||
[[Category: Dhote | |||
[[Category: | ==See Also== | ||
[[Category: Hashem | *[[Eukaryotic initiation factor 3D structures|Eukaryotic initiation factor 3D structures]] | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
</SX> | |||
[[Category: Large Structures]] | |||
[[Category: Oryctolagus cuniculus]] | |||
[[Category: Dhote V]] | |||
[[Category: Frank J]] | |||
[[Category: Hashem Y]] | |||
[[Category: Hellen CUT]] | |||
[[Category: Kuhn L]] | |||
[[Category: Pestova TV]] | |||
[[Category: Des-Georges A]] | |||
Latest revision as of 11:37, 9 May 2024
Structure of mammalian eIF3 in the context of the 43S preinitiation complex
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