5a95: Difference between revisions

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New page: '''Unreleased structure''' The entry 5a95 is ON HOLD Authors: Sulzenbacher, G., Lafond, M., Frey, T., Henrissat, B., Coutinho, R.M., Berrin, J.G., Garron, M.L. Description: CRYSTAL STR...
 
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'''Unreleased structure'''


The entry 5a95 is ON HOLD
==Crystal structure of beta-glucanase SdGluc5_26A from Saccharophagus degradans in complex with tetrasaccharide A, form 2==
<StructureSection load='5a95' size='340' side='right'caption='[[5a95]], [[Resolution|resolution]] 1.35&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5a95]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharophagus_degradans_2-40 Saccharophagus degradans 2-40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5A95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5A95 FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.35&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5a95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5a95 OCA], [https://pdbe.org/5a95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5a95 RCSB], [https://www.ebi.ac.uk/pdbsum/5a95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5a95 ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/Q21KE5_SACD2 Q21KE5_SACD2]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
In the CAZy database, glycoside hydrolase family 5 (GH5) is a large family with more than 6,000 sequences. Among the 51 described GH5 subfamilies, subfamily GH5_26 contains members that display either endo- beta(1,4)-glucanase or beta(1,3;1,4)-glucanase activities. In this study, we focused on the GH5_26 enzyme from Saccharophagus degradans (SdGluc5_26A) a marine bacterium known for its capacity to degrade a wide diversity of complex polysaccharides. SdGluc5_26A displays lichenase activity towards beta(1,3;1,4)-glucans with a side cellobiohydrolase activity towards beta(1,4)-glucans. The 3D structure of SdGluc5_26A adopts a stable trimeric quaternary structure observable also in solution. The N-terminal region of SdGluc5_26A protrudes into the active site of an adjacent monomer. To understand whether this occupation of the active site could influence its activity, we conducted a comprehensive enzymatic characterization of SdGluc5_26A and of a mutant truncated at the N-terminus. Ligand complex structures and kinetic analyses reveal that the N-terminus governs the substrate specificity of SdGluc5_26A. Its deletion opens the enzyme cleft at the -3 subsite and turns the enzyme into an endo-beta(1,4)-glucanase. This study demonstrates that experimental approaches can reveal structure-function relationships out of reach of current bioinformatic predictions.


Authors: Sulzenbacher, G., Lafond, M., Frey, T., Henrissat, B., Coutinho, R.M., Berrin, J.G., Garron, M.L.
the quaternary structure of a glycoside hydrolase dictates specificity towards beta-glucans.,Lafond M, Sulzenbacher G, Freyd T, Henrissat B, Berrin JG, Garron ML J Biol Chem. 2016 Jan 11. pii: jbc.M115.695999. PMID:26755730<ref>PMID:26755730</ref>


Description: CRYSTAL STRUCTURE BETA-GLUCANASE SDGLUC5_26A FROM SACCHAROPHAGUS DEGRADANS IN COMPLEX WITH TETRASACCHARIDE A, form 2
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Coutinho, R.M]]
<div class="pdbe-citations 5a95" style="background-color:#fffaf0;"></div>
[[Category: Sulzenbacher, G]]
 
[[Category: Lafond, M]]
==See Also==
[[Category: Berrin, J.G]]
*[[Glucanase 3D structures|Glucanase 3D structures]]
[[Category: Frey, T]]
== References ==
[[Category: Henrissat, B]]
<references/>
[[Category: Garron, M.L]]
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Saccharophagus degradans 2-40]]
[[Category: Berrin JG]]
[[Category: Coutinho RM]]
[[Category: Freyd T]]
[[Category: Garron ML]]
[[Category: Henrissat B]]
[[Category: Lafond M]]
[[Category: Sulzenbacher G]]
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