5co8: Difference between revisions
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New page: '''Unreleased structure''' The entry 5co8 is ON HOLD until sometime in the future Authors: LIU, Y.J., Freeman, A.D.J., Declais, A.C., Wilson, J.W., Gartner, A., Lilley, D.M.J. Descript... |
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The | ==Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA and Mg2+ ion== | ||
<StructureSection load='5co8' size='340' side='right'caption='[[5co8]], [[Resolution|resolution]] 2.40Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5co8]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CO8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CO8 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5co8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5co8 OCA], [https://pdbe.org/5co8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5co8 RCSB], [https://www.ebi.ac.uk/pdbsum/5co8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5co8 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/G0RYN2_CHATD G0RYN2_CHATD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
We present the crystal structure of the junction-resolving enzyme GEN1 bound to DNA at 2.5 A resolution. The structure of the GEN1 protein reveals it to have an elaborated FEN-XPG family fold that is modified for its role in four-way junction resolution. The functional unit in the crystal is a monomer of active GEN1 bound to the product of resolution cleavage, with an extensive DNA binding interface for both helical arms. Within the crystal lattice, a GEN1 dimer interface juxtaposes two products, whereby they can be reconnected into a four-way junction, the structure of which agrees with that determined in solution. The reconnection requires some opening of the DNA structure at the center, in agreement with permanganate probing and 2-aminopurine fluorescence. The structure shows that a relaxation of the DNA structure accompanies cleavage, suggesting how second-strand cleavage is accelerated to ensure productive resolution of the junction. | |||
Crystal Structure of a Eukaryotic GEN1 Resolving Enzyme Bound to DNA.,Liu Y, Freeman AD, Declais AC, Wilson TJ, Gartner A, Lilley DM Cell Rep. 2015 Dec 22;13(11):2565-75. doi: 10.1016/j.celrep.2015.11.042. Epub, 2015 Dec 10. PMID:26686639<ref>PMID:26686639</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5co8" style="background-color:#fffaf0;"></div> | ||
[[Category: Declais | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Chaetomium thermophilum var. thermophilum DSM 1495]] | ||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Declais AC]] | |||
[[Category: Freeman ADJ]] | |||
[[Category: Gartner A]] | |||
[[Category: Lilley DMJ]] | |||
[[Category: Liu YJ]] | |||
[[Category: Wilson TJ]] | |||
Latest revision as of 08:46, 23 October 2024
Crystal structure of the Holliday junction-resolving enzyme GEN1 (WT) in complex with product DNA and Mg2+ ion
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