5cse: Difference between revisions
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The | ==Streptavidin-S112Y-K121E Complexed with Palladium-Containing Biotin Ligand== | ||
<StructureSection load='5cse' size='340' side='right'caption='[[5cse]], [[Resolution|resolution]] 1.79Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5cse]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CSE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CSE FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.792Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SVP:CHLORO{DI-TERT-BUTYL[2-({5-[(3AS,4S,6AR)-2-OXOHEXAHYDRO-1H-THIENO[3,4-D]IMIDAZOL-4-YL]PENTANOYL}AMINO)ETHYL]-LAMBDA~5~-PHOSPHANYL}(1-PHENYLPROP-1-ENE-1,3-DIYL-KAPPA~2~C~1~,C~3~)PALLADIUM'>SVP</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cse FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cse OCA], [https://pdbe.org/5cse PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cse RCSB], [https://www.ebi.ac.uk/pdbsum/5cse PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cse ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Introduction of a biotinylated monophosphine palladium complex within streptavidin affords an enantioselective artificial Suzukiase. Site-directed mutagenesis allowed the optimization of the activity and the enantioselectivity of this artificial metalloenzyme. A variety of atropisomeric biaryls were produced in good yields and up to 90% ee. The hybrid catalyst described herein shows comparable TOF to the previous aqueous-asymmetric Suzuki catalysts, and excellent stability under the reaction conditions to realize higher TON through longer reaction time. | |||
An enantioselective artificial Suzukiase based on the biotin-streptavidin technology.,Chatterjee A, Mallin H, Klehr J, Vallapurackal J, Finke AD, Vera L, Marsh M, Ward TR Chem Sci. 2016 Jan 1;7(1):673-677. doi: 10.1039/c5sc03116h. Epub 2015 Oct 19. PMID:29896353<ref>PMID:29896353</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Chatterjee | <div class="pdbe-citations 5cse" style="background-color:#fffaf0;"></div> | ||
[[Category: Finke | |||
[[Category: | ==See Also== | ||
[[Category: | *[[Avidin 3D structures|Avidin 3D structures]] | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Streptomyces avidinii]] | |||
[[Category: Chatterjee A]] | |||
[[Category: Finke AD]] | |||
[[Category: Marsh M]] | |||
[[Category: Vera L]] | |||
[[Category: Ward TR]] | |||
