5cv2: Difference between revisions
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==Structural implications of homo-pyrimidine base pairs on the parallel-stranded d(GAY) motif== | |||
<StructureSection load='5cv2' size='340' side='right'caption='[[5cv2]], [[Resolution|resolution]] 2.69Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5cv2]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CV2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CV2 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.693Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SR:STRONTIUM+ION'>SR</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cv2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cv2 OCA], [https://pdbe.org/5cv2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cv2 RCSB], [https://www.ebi.ac.uk/pdbsum/5cv2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cv2 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
DNA can adopt many other structures beyond the canonical B-form double helix. There has been a growing interest in using non-canonical base pairs to provide structural diversity for designing DNA architectures for nanotechnology applications. We recently described the crystal structure of d(ACTCGGATGAT) which forms a tetraplex through parallel-stranded homobase pairs and nucleobase intercalation. Here, we examine the structural implications of the homo-pyrimidine base pair within this motif. We have determined crystal structures of two variants that differ from the original structure in the homo-pyrimidine base pairs and number of d(YGA) motifs. Our results show that the intercalation-locked tetraplex motif is predictable and that the substitution of C-C base pairs for T-T base pairs introduces asymmetry to the homoduplex. This hasimplications for utilizing d(YGA) motifs in DNA crystal design and may provide a basis for understanding local structures associated with repeat expansions. | |||
Structural implications of homo-pyrimidine base pairs in the parallel-stranded d(YGA) motif.,Tripathi S, Paukstelis PJ Chembiochem. 2015 Dec 2. doi: 10.1002/cbic.201500491. PMID:26629965<ref>PMID:26629965</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: Paukstelis | <div class="pdbe-citations 5cv2" style="background-color:#fffaf0;"></div> | ||
[[Category: Tripathi | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct]] | |||
[[Category: Paukstelis P]] | |||
[[Category: Tripathi S]] | |||
Latest revision as of 08:40, 27 September 2023
Structural implications of homo-pyrimidine base pairs on the parallel-stranded d(GAY) motif
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