5cbf: Difference between revisions
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==Structural and Functional Characterization of a Calcium-activated Cation channel from Tsukamurella Paurometabola== | |||
<StructureSection load='5cbf' size='340' side='right'caption='[[5cbf]], [[Resolution|resolution]] 3.61Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5cbf]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Tsukamurella_paurometabola_DSM_20162 Tsukamurella paurometabola DSM 20162]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CBF FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.61Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cbf OCA], [https://pdbe.org/5cbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cbf RCSB], [https://www.ebi.ac.uk/pdbsum/5cbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cbf ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/D5UM26_TSUPD D5UM26_TSUPD] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The selectivity filter is an essential functional element of K(+) channels that is highly conserved both in terms of its primary sequence and its three-dimensional structure. Here, we investigate the properties of an ion channel from the Gram-positive bacterium Tsukamurella paurometabola with a selectivity filter formed by an uncommon proline-rich sequence. Electrophysiological recordings show that it is a non-selective cation channel and that its activity depends on Ca(2+) concentration. In the crystal structure, the selectivity filter adopts a novel conformation with Ca(2+) ions bound within the filter near the pore helix where they are coordinated by backbone oxygen atoms, a recurrent motif found in multiple proteins. The binding of Ca(2+) ion in the selectivity filter controls the widening of the pore as shown in crystal structures and in molecular dynamics simulations. The structural, functional and computational data provide a characterization of this calcium-gated cationic channel. | |||
Structural and functional characterization of a calcium-activated cation channel from Tsukamurella paurometabola.,Dhakshnamoorthy B, Rohaim A, Rui H, Blachowicz L, Roux B Nat Commun. 2016 Sep 28;7:12753. doi: 10.1038/ncomms12753. PMID:27678077<ref>PMID:27678077</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5cbf" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Tsukamurella paurometabola DSM 20162]] | |||
[[Category: Blachowicz L]] | |||
[[Category: Dhakshnamoorthy B]] | |||
[[Category: Rohaim A]] | |||
[[Category: Roux B]] | |||
[[Category: Rui H]] | |||
