5d8n: Difference between revisions

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New page: '''Unreleased structure''' The entry 5d8n is ON HOLD Authors: DuPrez, K.T., Scranton, M.A., Walling, L.L., Fan, L. Description: Tomato leucine aminopeptidase mutant -K354E [[Category: ...
 
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'''Unreleased structure'''


The entry 5d8n is ON HOLD
==Tomato leucine aminopeptidase mutant - K354E==
<StructureSection load='5d8n' size='340' side='right'caption='[[5d8n]], [[Resolution|resolution]] 2.15&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5d8n]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Solanum_lycopersicum Solanum lycopersicum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5D8N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5D8N FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5d8n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5d8n OCA], [https://pdbe.org/5d8n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5d8n RCSB], [https://www.ebi.ac.uk/pdbsum/5d8n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5d8n ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/AMPL1_SOLLC AMPL1_SOLLC] Presumably involved in the processing and regular turnover of intracellular proteins.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Tomato plants express acidic leucine aminopeptidase (LAP-A) in response to various environmental stressors. LAP-A not only functions as a peptidase for diverse peptide substrates, but also displays chaperone activity. A K354E mutation has been shown to abolish the peptidase activity but to enhance the chaperone activity of LAP-A. To better understand this moonlighting function of LAP-A, the crystal structure of the K354E mutant was determined at 2.15 A resolution. The structure reveals that the K354E mutation destabilizes an active-site loop and causes significant rearrangement of active-site residues, leading to loss of the catalytic metal-ion coordination required for the peptidase activity. Although the mutant was crystallized in the same hexameric form as wild-type LAP-A, gel-filtration chromatography revealed an apparent shift from the hexamer to lower-order oligomers for the K354E mutant, showing a mixture of monomers to trimers in solution. In addition, surface-probing assays indicated that the K354E mutant has more accessible hydrophobic areas than wild-type LAP-A. Consistently, computational thermodynamic estimations of the interfaces between LAP-A monomers suggest that increased exposure of hydrophobic surfaces occurs upon hexamer breakdown. These results suggest that the K354E mutation disrupts the active-site loop, which also contributes to the hexameric assembly, and destabilizes the hexamers, resulting in much greater hydrophobic areas accessible for efficient chaperone activity than in the wild-type LAP-A.


Authors: DuPrez, K.T., Scranton, M.A., Walling, L.L., Fan, L.
Structural insights into chaperone-activity enhancement by a K354E mutation in tomato acidic leucine aminopeptidase.,DuPrez KT, Scranton MA, Walling LL, Fan L Acta Crystallogr D Struct Biol. 2016 May;72(Pt 5):694-702. doi:, 10.1107/S205979831600509X. Epub 2016 Apr 26. PMID:27139632<ref>PMID:27139632</ref>


Description: Tomato leucine aminopeptidase mutant -K354E
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Fan, L]]
<div class="pdbe-citations 5d8n" style="background-color:#fffaf0;"></div>
[[Category: Walling, L.L]]
 
[[Category: Scranton, M.A]]
==See Also==
[[Category: Duprez, K.T]]
*[[Aminopeptidase 3D structures|Aminopeptidase 3D structures]]
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Solanum lycopersicum]]
[[Category: DuPrez KT]]
[[Category: Fan L]]
[[Category: Scranton MA]]
[[Category: Walling LL]]
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