4v8v

Structure and conformational variability of the Mycobacterium tuberculosis fatty acid synthase multienzyme complex

Loading... Status: waiting for Mol* initialization Structural highlights 4v8v is a 6 chain structure with sequence from Mycobacterium tuberculosis. This structure supersedes the now removed PDB entries 4bjd and 4bje. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance. Method: Electron Microscopy, Resolution 20Å Ligands: FMN Resources: FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT Publication Abstract from PubMed Antibiotic therapy in response to Mycobacterium tuberculosis infections targets de novo fatty acid biosynthesis, which is orchestrated by a 1.9 MDa type I fatty acid synthase (FAS). Here, we characterize M. tuberculosis FAS by single-particle cryo-electron microscopy and interpret the data by docking the molecular models of yeast and Mycobacterium smegmatis FAS. Our analysis reveals a porous barrel-like structure of considerable conformational variability that is illustrated by the identification of several conformational states with altered topology in the multienzymatic assembly. This demonstrates that the barrel-like structure of M. tuberculosis FAS is not just a static scaffold for the catalytic domains, but may play an active role in coordinating fatty acid synthesis. The conception of M. tuberculosis FAS as a highly dynamic assembly of domains revises the view on bacterial type I fatty acid synthesis and might inspire new strategies for inhibition of de novo fatty acid synthesis in M. tuberculosis. Structure and Conformational Variability of the Mycobacterium tuberculosis Fatty Acid Synthase Multienzyme Complex.,Ciccarelli L, Connell SR, Enderle M, Mills DJ, Vonck J, Grininger M Structure. 2013 Jun 5. pii: S0969-2126(13)00153-6. doi:, 10.1016/j.str.2013.04.023. PMID:23746808 From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

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