5dac: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(5 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''Unreleased structure'''


The entry 5dac is ON HOLD  until Paper Publication
==ATP-gamma-S bound Rad50 from Chaetomium thermophilum in complex with DNA==
<StructureSection load='5dac' size='340' side='right'caption='[[5dac]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5dac]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DAC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DAC FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.503&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AES:4-(2-AMINOETHYL)BENZENESULFONYL+FLUORIDE'>AES</scene>, <scene name='pdbligand=AGS:PHOSPHOTHIOPHOSPHORIC+ACID-ADENYLATE+ESTER'>AGS</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dac FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dac OCA], [https://pdbe.org/5dac PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dac RCSB], [https://www.ebi.ac.uk/pdbsum/5dac PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dac ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/G0SHW7_CHATD G0SHW7_CHATD]
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The Mre11-Rad50-Nbs1 (MRN) complex is a central factor in the repair of DNA double-strand breaks (DSBs). The ATP-dependent mechanisms of how MRN detects and endonucleolytically processes DNA ends for the repair by microhomology-mediated end-joining or further resection in homologous recombination are still unclear. Here, we report the crystal structures of the ATPgammaS-bound dimer of the Rad50NBD (nucleotide-binding domain) from the thermophilic eukaryote Chaetomium thermophilum (Ct) in complex with either DNA or CtMre11RBD (Rad50-binding domain) along with small-angle X-ray scattering and cross-linking studies. The structure and DNA binding motifs were validated by DNA binding experiments in vitro and mutational analyses in Saccharomyces cerevisiae in vivo. Our analyses provide a structural framework for the architecture of the eukaryotic Mre11-Rad50 complex. They show that a Rad50 dimer binds approximately 18 base pairs of DNA along the dimer interface in an ATP-dependent fashion or bridges two DNA ends with a preference for 3' overhangs. Finally, our results may provide a general framework for the interaction of ABC ATPase domains of the Rad50/SMC/RecN protein family with DNA.


Authors: Seifert, F.U., Lammens, K., Stoehr, G., Kessler, B., Hopfner, K.-P.
Structural mechanism of ATP-dependent DNA binding and DNA end bridging by eukaryotic Rad50.,Seifert FU, Lammens K, Stoehr G, Kessler B, Hopfner KP EMBO J. 2016 Feb 19. pii: e201592934. PMID:26896444<ref>PMID:26896444</ref>


Description:  
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Hopfner, K.-P]]
<div class="pdbe-citations 5dac" style="background-color:#fffaf0;"></div>
[[Category: Stoehr, G]]
== References ==
[[Category: Lammens, K]]
<references/>
[[Category: Kessler, B]]
__TOC__
[[Category: Seifert, F.U]]
</StructureSection>
[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
[[Category: Large Structures]]
[[Category: Synthetic construct]]
[[Category: Hopfner K-P]]
[[Category: Kessler B]]
[[Category: Lammens K]]
[[Category: Seifert FU]]
[[Category: Stoehr G]]
Retrieved from "https://proteopedia.org/5dac"