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==Crystal structure of calcium-loaded apo-obelin from Obelia longissima==
==Crystal structure of calcium-loaded apo-obelin from Obelia longissima==
<StructureSection load='1sl7' size='340' side='right' caption='[[1sl7]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='1sl7' size='340' side='right'caption='[[1sl7]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[1sl7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Black_sea_hydrozoan Black sea hydrozoan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SL7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1SL7 FirstGlance]. <br>
<table><tr><td colspan='2'>[[1sl7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Obelia_longissima Obelia longissima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1SL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1SL7 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1el4|1el4]], [[1ej3|1ej3]], [[1jf0|1jf0]], [[1jf2|1jf2]], [[1qv0|1qv0]], [[1qv1|1qv1]], [[1s36|1s36]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1sl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sl7 OCA], [http://pdbe.org/1sl7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1sl7 RCSB], [http://www.ebi.ac.uk/pdbsum/1sl7 PDBsum], [http://www.topsan.org/Proteins/SECSG/1sl7 TOPSAN]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1sl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1sl7 OCA], [https://pdbe.org/1sl7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1sl7 RCSB], [https://www.ebi.ac.uk/pdbsum/1sl7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1sl7 ProSAT], [https://www.topsan.org/Proteins/SECSG/1sl7 TOPSAN]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/OBL_OBELO OBL_OBELO]] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.  
[https://www.uniprot.org/uniprot/OBL_OBELO OBL_OBELO] Ca(2+)-dependent bioluminescence photoprotein. Displays an emission peak at 470 nm (blue light). Trace amounts of calcium ion trigger the intramolecular oxidation of the chromophore, coelenterazine into coelenteramide and CO(2) with the concomitant emission of light.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
Check<jmol>
   <jmolCheckbox>
   <jmolCheckbox>
     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sl/1sl7_consurf.spt"</scriptWhenChecked>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/sl/1sl7_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
   </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1sl7 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Black sea hydrozoan]]
[[Category: Large Structures]]
[[Category: Deng, L]]
[[Category: Obelia longissima]]
[[Category: Lee, J]]
[[Category: Deng L]]
[[Category: Liu, Z J]]
[[Category: Lee J]]
[[Category: Markova, S V]]
[[Category: Liu ZJ]]
[[Category: Rose, J]]
[[Category: Markova SV]]
[[Category: Structural genomic]]
[[Category: Rose J]]
[[Category: Vysotski, E S]]
[[Category: Vysotski ES]]
[[Category: Wang, B C]]
[[Category: Wang BC]]
[[Category: Aequorin]]
[[Category: Bioluminescence]]
[[Category: Calcium binding]]
[[Category: Ef-hand]]
[[Category: Fluorescence]]
[[Category: Luminescent protein]]
[[Category: Obelin]]
[[Category: Photoprotein]]
[[Category: PSI, Protein structure initiative]]
[[Category: Secsg]]

Latest revision as of 06:18, 23 August 2023

Crystal structure of calcium-loaded apo-obelin from Obelia longissima

1sl7, resolution 2.20Å

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