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[[Image:2gek.jpg|left|200px]]


{{Structure
==Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP==
|PDB= 2gek |SIZE=350|CAPTION= <scene name='initialview01'>2gek</scene>, resolution 2.400&Aring;
<StructureSection load='2gek' size='340' side='right'caption='[[2gek]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5&#39;-DIPHOSPHATE'>GDP</scene>
<table><tr><td colspan='2'>[[2gek]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycolicibacterium_smegmatis_MC2_155 Mycolicibacterium smegmatis MC2 155]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GEK FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4&#8491;</td></tr>
|GENE= PIMA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1772 Mycobacterium smegmatis])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr>
}}
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gek FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gek OCA], [https://pdbe.org/2gek PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gek RCSB], [https://www.ebi.ac.uk/pdbsum/2gek PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gek ProSAT]</span></td></tr>
 
</table>
'''Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP'''
== Function ==
 
[https://www.uniprot.org/uniprot/PIMA_MYCS2 PIMA_MYCS2] Catalyzes the addition of a mannose residue from GDP-D-mannose to the position 2 of a phosphatidyl-myo-inositol (PI) to generate a phosphatidyl-myo-inositol bearing an alpha-1,2-linked mannose residue (PIM1). Contrary to PimB, the mannosyltransferase PimA is unable to transfer a mannose residue to the position 6 of the phosphatidyl-myo-inositola of PIM1.<ref>PMID:12068013</ref> <ref>PMID:19638342</ref>
 
== Evolutionary Conservation ==
==Overview==
[[Image:Consurf_key_small.gif|200px|right]]
Phosphatidylinositol mannosyltransferase (PimA) is an essential enzyme for mycobacterial growth that catalyses the first mannosylation step in phosphatidyl-myo-inositol mannoside (PIM) biosynthesis. The enzyme belongs to the large GT4 family of glycosyltransferases, for which no structure is currently available. Recombinant purified PimA from Mycobacterium smegmatis has been crystallized in the presence of GDP and myo-inositol. The crystals belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.2, b = 72.4, c = 138.2 A, and diffract to 2.4 A resolution.
Check<jmol>
 
  <jmolCheckbox>
==About this Structure==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ge/2gek_consurf.spt"</scriptWhenChecked>
2GEK is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Mycobacterium_smegmatis Mycobacterium smegmatis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GEK OCA].  
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==Reference==
  </jmolCheckbox>
Crystallization and preliminary crystallographic analysis of PimA, an essential mannosyltransferase from Mycobacterium smegmatis., Guerin ME, Buschiazzo A, Kordulakova J, Jackson M, Alzari PM, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 May 1;61(Pt, 5):518-20. Epub 2005 Apr 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511084 16511084]
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gek ConSurf].
[[Category: Mycobacterium smegmatis]]
<div style="clear:both"></div>
[[Category: Protein complex]]
== References ==
[[Category: Alzari, P M.]]
<references/>
[[Category: Buschiazzo, A.]]
__TOC__
[[Category: Guerin, M E.]]
</StructureSection>
[[Category: Jackson, M.]]
[[Category: Large Structures]]
[[Category: Kordulakova, J.]]
[[Category: Mycolicibacterium smegmatis MC2 155]]
[[Category: GDP]]
[[Category: Alzari PM]]
[[Category: binary complex]]
[[Category: Buschiazzo A]]
[[Category: gt4 glycosyltransferase]]
[[Category: Guerin ME]]
[[Category: mannosyltransferase]]
[[Category: Jackson M]]
[[Category: rossmann fold]]
[[Category: Kordulakova J]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 23 15:10:19 2008''

Latest revision as of 09:27, 14 February 2024

Crystal Structure of phosphatidylinositol mannosyltransferase (PimA) from Mycobacterium smegmatis in complex with GDP

2gek, resolution 2.40Å

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