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==Crystal structure of the Rho-GTPase binding domain of YpkA==
==Crystal structure of the Rho-GTPase binding domain of YpkA==
<StructureSection load='2h7o' size='340' side='right' caption='[[2h7o]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='2h7o' size='340' side='right'caption='[[2h7o]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2h7o]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_pseudotuberkulosis"_(sic)_pfeiffer_1889 "bacillus pseudotuberkulosis" (sic) pfeiffer 1889]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7O OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2H7O FirstGlance]. <br>
<table><tr><td colspan='2'>[[2h7o]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Yersinia_pseudotuberculosis Yersinia pseudotuberculosis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2H7O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2H7O FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ypkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=633 "Bacillus pseudotuberkulosis" (sic) Pfeiffer 1889])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7o OCA], [http://pdbe.org/2h7o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2h7o RCSB], [http://www.ebi.ac.uk/pdbsum/2h7o PDBsum]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2h7o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7o OCA], [https://pdbe.org/2h7o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2h7o RCSB], [https://www.ebi.ac.uk/pdbsum/2h7o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2h7o ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/YPKA_YERPS YPKA_YERPS]] Acts as a virulence determinant.<ref>PMID:10920208</ref> <ref>PMID:17531806</ref> 
[https://www.uniprot.org/uniprot/YPKA_YERPS YPKA_YERPS] Acts as a virulence determinant.<ref>PMID:10920208</ref> <ref>PMID:17531806</ref>  
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. A critical virulence determinant is the Yersinia protein kinase A, or YpkA, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a Rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface abolish this activity in vitro and impair in vivo YpkA-induced cytoskeletal disruption. In cell culture experiments, the kinase and the GDI domains of YpkA act synergistically to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity shows attenuated virulence in a mouse infection assay. We conclude that virulence in Yersinia depends strongly upon mimicry of host GDI proteins by YpkA.
 
Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors.,Prehna G, Ivanov MI, Bliska JB, Stebbins CE Cell. 2006 Sep 8;126(5):869-80. PMID:16959567<ref>PMID:16959567</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2h7o" style="background-color:#fffaf0;"></div>
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bliska, J B]]
[[Category: Large Structures]]
[[Category: Ivanov, M]]
[[Category: Yersinia pseudotuberculosis]]
[[Category: Prehna, G]]
[[Category: Bliska JB]]
[[Category: Stebbins, C E]]
[[Category: Ivanov M]]
[[Category: Gdi]]
[[Category: Prehna G]]
[[Category: Signaling protein]]
[[Category: Stebbins CE]]
[[Category: Yersinia]]
[[Category: Yopo]]
[[Category: Ypka]]
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