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==Crystal Structure and Functional Studies Reveal that PAS Factor from Vibrio vulnificus is a Novel Member of the Saposin-Fold Family==
==Crystal Structure and Functional Studies Reveal that PAS Factor from Vibrio vulnificus is a Novel Member of the Saposin-Fold Family==
<StructureSection load='2b8i' size='340' side='right' caption='[[2b8i]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='2b8i' size='340' side='right'caption='[[2b8i]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2b8i]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_27562 Atcc 27562]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B8I OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B8I FirstGlance]. <br>
<table><tr><td colspan='2'>[[2b8i]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_vulnificus Vibrio vulnificus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B8I OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2B8I FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b8i OCA], [http://pdbe.org/2b8i PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b8i RCSB], [http://www.ebi.ac.uk/pdbsum/2b8i PDBsum]</span></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2b8i FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b8i OCA], [https://pdbe.org/2b8i PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2b8i RCSB], [https://www.ebi.ac.uk/pdbsum/2b8i PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2b8i ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/Q56GA4_VIBVL Q56GA4_VIBVL]
PAS factor is a novel putative bacterial secretion factor thought to induce secretion of periplasmic proteins. We solved the crystal structure of PAS factor from Vibrio vulnificus at 1.8A resolution and found it to be comprised of five alpha helices that form an antiparallel bundle with an up-and-down topology, and to adopt the saposin-fold characteristic of a family of proteins that bind to membranes and lipids. PAS factor lacks the disulfide bridge characteristic of mammalian saposin-fold proteins; in fact, it shows no sequence homology with mammalian proteins. Nevertheless, the molecular architectures are similar, and the shared propensity for membrane interaction suggests strongly that PAS factor is another member of the saposin-fold family. Analysis of the CD spectra showed that PAS factor binds to membranes directly, while measurement of calcein dye leakage showed that PAS factor interacts strongly with liposomes composed of anionic phospholipids, making them leaky, but binds very weakly with liposomes composed of zwitterionic phospholipids. Moreover, by analyzing tryptophan fluorescence emission from four single-tryptophan mutants (V10W, T22W, F35W, and L70W), we identified the putative phospholipid-binding site of PAS factor. The resultant membrane destabilization likely mediates secretion of periplasmic proteins required for the in vivo survival and pathogenesis of V.vulnificus.
 
Crystal structure and functional studies reveal that PAS factor from Vibrio vulnificus is a novel member of the saposin-fold family.,Lee JH, Yang ST, Rho SH, Im YJ, Kim SY, Kim YR, Kim MK, Kang GB, Kim JI, Rhee JH, Eom SH J Mol Biol. 2006 Jan 20;355(3):491-500. Epub 2005 Nov 14. PMID:16318855<ref>PMID:16318855</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2b8i" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Atcc 27562]]
[[Category: Large Structures]]
[[Category: Eom, S H]]
[[Category: Vibrio vulnificus]]
[[Category: Im, Y J]]
[[Category: Eom SH]]
[[Category: Kang, G B]]
[[Category: Im YJ]]
[[Category: Kim, J I]]
[[Category: Kang GB]]
[[Category: Kim, M K]]
[[Category: Kim JI]]
[[Category: Kim, S Y]]
[[Category: Kim MK]]
[[Category: Kim, Y R]]
[[Category: Kim SY]]
[[Category: Lee, J H]]
[[Category: Kim YR]]
[[Category: Rhee, J H]]
[[Category: Lee JH]]
[[Category: Rho, S H]]
[[Category: Rhee JH]]
[[Category: Yang, S T]]
[[Category: Rho SH]]
[[Category: Four helix bundle]]
[[Category: Yang ST]]
[[Category: Lipid binding protein]]

Latest revision as of 13:42, 13 March 2024

Crystal Structure and Functional Studies Reveal that PAS Factor from Vibrio vulnificus is a Novel Member of the Saposin-Fold Family

2b8i, resolution 1.80Å

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