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| ==Solution structure of P2a-J2a/b-P2b of human telomerase RNA== | | ==Solution structure of P2a-J2a/b-P2b of human telomerase RNA== |
| <StructureSection load='2l3e' size='340' side='right' caption='[[2l3e]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | | <StructureSection load='2l3e' size='340' side='right'caption='[[2l3e]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[2l3e]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L3E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L3E FirstGlance]. <br> | | <table><tr><td colspan='2'>[[2l3e]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L3E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L3E FirstGlance]. <br> |
| </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3e OCA], [http://pdbe.org/2l3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l3e RCSB], [http://www.ebi.ac.uk/pdbsum/2l3e PDBsum]</span></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l3e OCA], [https://pdbe.org/2l3e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l3e RCSB], [https://www.ebi.ac.uk/pdbsum/2l3e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l3e ProSAT]</span></td></tr> |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
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| == Publication Abstract from PubMed ==
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| Telomerase is a unique ribonucleoprotein complex that catalyzes the addition of telomeric DNA repeats onto the 3' ends of linear chromosomes. All vertebrate telomerase RNAs contain a catalytically essential core domain that includes the template and a pseudoknot with extended helical subdomains. Within these helical regions is an asymmetric 5-nt internal bulge loop (J2a/b) flanked by helices (P2a and P2b) that is highly conserved in its location but not sequence. NMR structure determination reveals that J2a/b forms a defined S-shape and creates an approximately 90 degrees bend with a surprisingly low twist ( approximately 10 degrees ) between the flanking helices. A search of RNA structures revealed only one other example of a 5-nt bulge, from hepatitis C virus internal ribosome entry site, with a different sequence but the same structure. J2a/b is intrinsically flexible but the interhelical motions across the loop are remarkably restricted. Nucleotide substitutions in J2a/b that affect the bend angle, direction, and interhelical dynamics are correlated with telomerase activity. Based on the structures of P2ab (J2a/b and flanking helices), the conserved region of the pseudoknot (P2b/P3, previously determined) and the remaining helical segment (P2a.1-J2a.1 refined using residual dipolar couplings and the modeling program MC-Sym) we have calculated an NMR-based model of the full-length pseudoknot. The model and dynamics analysis show that J2a/b serves as a dominant structural and dynamical element in defining the overall topology of the core domain, and suggest that interhelical motions in P2ab facilitate nucleotide addition along the template and template translocation.
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| Structurally conserved five nucleotide bulge determines the overall topology of the core domain of human telomerase RNA.,Zhang Q, Kim NK, Peterson RD, Wang Z, Feigon J Proc Natl Acad Sci U S A. 2010 Nov 2;107(44):18761-8. Epub 2010 Oct 21. PMID:20966348<ref>PMID:20966348</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| | ==See Also== |
| </div>
| | *[[Telomerase 3D structures|Telomerase 3D structures]] |
| <div class="pdbe-citations 2l3e" style="background-color:#fffaf0;"></div>
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| == References == | |
| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Feigon, J]] | | [[Category: Large Structures]] |
| [[Category: Kim, N]] | | [[Category: Feigon J]] |
| [[Category: Peterson, R D]] | | [[Category: Kim N]] |
| [[Category: Wang, Z]] | | [[Category: Peterson RD]] |
| [[Category: Zhang, Q]] | | [[Category: Wang Z]] |
| [[Category: Htr]]
| | [[Category: Zhang Q]] |
| [[Category: Human telomerase rna]]
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| [[Category: J2ab]]
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| [[Category: P2a]]
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| [[Category: P2b]]
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| [[Category: Rna]]
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