2kbz: Difference between revisions

Latest revision as of 19:11, 29 May 2024

NMR structure of protein gp15 of bacteriophage SPP1

Structural highlights

2kbz is a 1 chain structure with sequence from Bacillus phage SPP1. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	Solution NMR
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HCP15_BPSPP Head completion protein that exhibits an open central channel for viral DNA ejection. Part of the head-tail connector by binding to the portal protein and to the head completion protein 16.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

In many bacterial viruses and in certain animal viruses, the double-stranded DNA genome enters and exits the capsid through a portal gatekeeper. We report a pseudoatomic structure of a complete portal system. The bacteriophage SPP1 gatekeeper is composed of dodecamers of the portal protein gp6, the adaptor gp15, and the stopper gp16. The solution structures of gp15 and gp16 were determined by NMR. They were then docked together with the X-ray structure of gp6 into the electron density of the approximately 1-MDa SPP1 portal complex purified from DNA-filled capsids. The resulting structure reveals that gatekeeper assembly is accompanied by a large rearrangement of the gp15 structure and by folding of a flexible loop of gp16 to form an intersubunit parallel beta-sheet that closes the portal channel. This stopper system prevents release of packaged DNA. Disulfide cross-linking between beta-strands of the stopper blocks the key conformational changes that control genome ejection from the virus at the beginning of host infection.

Structure of bacteriophage SPP1 head-to-tail connection reveals mechanism for viral DNA gating.,Lhuillier S, Gallopin M, Gilquin B, Brasiles S, Lancelot N, Letellier G, Gilles M, Dethan G, Orlova EV, Couprie J, Tavares P, Zinn-Justin S Proc Natl Acad Sci U S A. 2009 May 26;106(21):8507-12. Epub 2009 May 11. PMID:19433794^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chaban Y, Lurz R, Brasiles S, Cornilleau C, Karreman M, Zinn-Justin S, Tavares P, Orlova EV. Structural rearrangements in the phage head-to-tail interface during assembly and infection. Proc Natl Acad Sci U S A. 2015 May 19. pii: 201504039. PMID:25991862 doi:https://dx.doi.org/10.1073/pnas.1504039112
↑ Lhuillier S, Gallopin M, Gilquin B, Brasiles S, Lancelot N, Letellier G, Gilles M, Dethan G, Orlova EV, Couprie J, Tavares P, Zinn-Justin S. Structure of bacteriophage SPP1 head-to-tail connection reveals mechanism for viral DNA gating. Proc Natl Acad Sci U S A. 2009 May 26;106(21):8507-12. Epub 2009 May 11. PMID:19433794

Proteopedia Page Contributors and Editors (what is this?)

OCA

[1] Chaban Y, Lurz R, Brasiles S, Cornilleau C, Karreman M, Zinn-Justin S, Tavares P, Orlova EV. Structural rearrangements in the phage head-to-tail interface during assembly and infection. Proc Natl Acad Sci U S A. 2015 May 19. pii: 201504039. PMID:25991862 doi:https://dx.doi.org/10.1073/pnas.1504039112

[2] Lhuillier S, Gallopin M, Gilquin B, Brasiles S, Lancelot N, Letellier G, Gilles M, Dethan G, Orlova EV, Couprie J, Tavares P, Zinn-Justin S. Structure of bacteriophage SPP1 head-to-tail connection reveals mechanism for viral DNA gating. Proc Natl Acad Sci U S A. 2009 May 26;106(21):8507-12. Epub 2009 May 11. PMID:19433794

[1]

[2]

@@ Line 1: / Line 1: @@
 ==NMR structure of protein gp15 of bacteriophage SPP1==
-<StructureSection load='2kbz' size='340' side='right' caption='[[2kbz]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
+<StructureSection load='2kbz' size='340' side='right'caption='[[2kbz]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[2kbz]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bpspp Bpspp]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBZ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KBZ FirstGlance]. <br>
+<table><tr><td colspan='2'>[[2kbz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_phage_SPP1 Bacillus phage SPP1]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KBZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KBZ FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kbz OCA], [http://pdbe.org/2kbz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kbz RCSB], [http://www.ebi.ac.uk/pdbsum/2kbz PDBsum]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kbz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kbz OCA], [https://pdbe.org/2kbz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kbz RCSB], [https://www.ebi.ac.uk/pdbsum/2kbz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kbz ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/HCP15_BPSPP HCP15_BPSPP] Head completion protein that exhibits an open central channel for viral DNA ejection. Part of the head-tail connector by binding to the portal protein and to the head completion protein 16.<ref>PMID:25991862</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
    <jmolCheckbox>
-     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/2kbz_consurf.spt"</scriptWhenChecked>
+     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/kb/2kbz_consurf.spt"</scriptWhenChecked>
      <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
    </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2kbz ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">
@@ Line 28: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Bpspp]]
+[[Category: Bacillus phage SPP1]]
-[[Category: Gallopin, M]]
+[[Category: Large Structures]]
-[[Category: Gilquin, B]]
+[[Category: Gallopin M]]
-[[Category: Zinn-Justin, S]]
+[[Category: Gilquin B]]
-[[Category: Three alpha-helix bundle]]
+[[Category: Zinn-Justin S]]
-[[Category: Viral protein]]

2kbz: Difference between revisions

Latest revision as of 19:11, 29 May 2024

NMR structure of protein gp15 of bacteriophage SPP1

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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