5dol: Difference between revisions

From Proteopedia
Jump to navigationJump to search
← Older edit
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
m Protected "5dol" [edit=sysop:move=sysop]
OCA (talk | contribs)
Bots, Bureaucrats, journal, Administrators
3,328,572 edits
No edit summary
 
(3 intermediate revisions by the same user not shown)
Line 1: Line 1:
'''Unreleased structure'''


The entry 5dol is ON HOLD  until Paper Publication
==Crystal structure of YabA amino-terminal domain from Bacillus subtilis==
<StructureSection load='5dol' size='340' side='right'caption='[[5dol]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[5dol]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis_subsp._subtilis_str._168 Bacillus subtilis subsp. subtilis str. 168]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5DOL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5DOL FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5dol FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5dol OCA], [https://pdbe.org/5dol PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5dol RCSB], [https://www.ebi.ac.uk/pdbsum/5dol PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5dol ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/YABA_BACSU YABA_BACSU] Involved in initiation control of chromosome replication.<ref>PMID:12060778</ref>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
YabA negatively regulates initiation of DNA replication in low-GC Gram-positive bacteria. The protein exerts its control through interactions with the initiator protein DnaA and the sliding clamp DnaN. Here, we combined X-ray crystallography, X-ray scattering (SAXS), modeling and biophysical approaches, with in vivo experimental data to gain insight into YabA function. The crystal structure of the N-terminal domain (NTD) of YabA solved at 2.7 A resolution reveals an extended alpha-helix that contributes to an intermolecular four-helix bundle. Homology modeling and biochemical analysis indicates that the C-terminal domain (CTD) of YabA is a small Zn-binding domain. Multi-angle light scattering and SAXS demonstrate that YabA is a tetramer in which the CTDs are independent and connected to the N-terminal four-helix bundle via flexible linkers. While YabA can simultaneously interact with both DnaA and DnaN, we found that an isolated CTD can bind to either DnaA or DnaN, individually. Site-directed mutagenesis and yeast-two hybrid assays identified DnaA and DnaN binding sites on the YabA CTD that partially overlap and point to a mutually exclusive mode of interaction. Our study defines YabA as a novel structural hub and explains how the protein tetramer uses independent CTDs to bind multiple partners to orchestrate replication initiation in the bacterial cell.


Authors: Cherrier, M.V., Bazin, A., Jameson, K.H., Wilkinson, A.J., Noirot-Gros, M.F., Terradot, L.
Tetramerization and interdomain flexibility of the replication initiation controller YabA enables simultaneous binding to multiple partners.,Felicori L, Jameson KH, Roblin P, Fogg MJ, Garcia-Garcia T, Ventroux M, Cherrier MV, Bazin A, Noirot P, Wilkinson AJ, Molina F, Terradot L, Noirot-Gros MF Nucleic Acids Res. 2016 Jan 8;44(1):449-63. doi: 10.1093/nar/gkv1318. Epub 2015, Nov 28. PMID:26615189<ref>PMID:26615189</ref>


Description: Crystal structure of YabA amino-terminal domain from Bacillus subtilis
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
[[Category: Unreleased Structures]]
</div>
[[Category: Noirot-Gros, M.F]]
<div class="pdbe-citations 5dol" style="background-color:#fffaf0;"></div>
[[Category: Wilkinson, A.J]]
== References ==
[[Category: Bazin, A]]
<references/>
[[Category: Terradot, L]]
__TOC__
[[Category: Jameson, K.H]]
</StructureSection>
[[Category: Cherrier, M.V]]
[[Category: Bacillus subtilis subsp. subtilis str. 168]]
[[Category: Large Structures]]
[[Category: Bazin A]]
[[Category: Cherrier MV]]
[[Category: Jameson KH]]
[[Category: Noirot-Gros MF]]
[[Category: Terradot L]]
[[Category: Wilkinson AJ]]
Retrieved from "https://proteopedia.org/5dol"